ARR12 promotes de novo shoot regeneration in Arabidopsis thaliana via activation of WUSCHEL expression
ARR12 promotes de novo shoot regeneration in Arabidopsis thaliana via activation of WUSCHEL expression
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ARR12 通过激活 WUSCHEL 表达促进拟南芥芽再生
DOI：
10.1111/jipb.12567
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发表时间：
2017-10-01
影响因子：
11.4
通讯作者：
Xiang, Fengning
中科院分区：
文献类型：
--
作者：
Dai, Xuehuan;Liu, Zhenhua;Xiang, Fengning
生长素和细胞分裂素在植物细胞的体外培养过程中指导细胞增殖和分化，但这些过程的分子基础，特别是从头芽再生，尚未完全阐明。在这里，我们描述了拟南芥（L.）Heynh，基于拟南芥应答调节因子12（ARR12）和WUSCHEL（WUS）的相互作用。ARR12的主要表达位点与茎尖分生组织（SAM）起始的位置一致。arr12突变体表现出严重受损的芽再生和细胞分裂素的反应性降低，与此一致，ARR12的过表达增强芽再生。某些芽分生组织特化基因，特别是WUSCHEL（WUS）和CLAVATA 3，在arr12外植体中显著下调。染色质免疫沉淀（ChIP）和瞬时激活试验表明，ARR12结合WUS的启动子。这些观察结果表明，在芽再生过程中，在体外，ARR12的功能作为细胞分裂素信号传导和芽分生组织特化基因的表达之间的分子联系。
Auxin and cytokinin direct cell proliferation and differentiation during the in vitro culture of plant cells, but the molecular basis of these processes, especially de novo shoot regeneration, has not been fully elucidated. Here, we describe the regulatory control of shoot regeneration in Arabidopsis thaliana (L.) Heynh, based on the interaction of ARABIDOPSIS RESPONSE REGULATOR12 (ARR12) and WUSCHEL (WUS). The major site of ARR12 expression coincided with the location where the shoot apical meristem (SAM) initiated. The arr12 mutants showed severely impaired shoot regeneration and reduced responsiveness to cytokinin; consistent with this, the overexpression of ARR12 enhanced shoot regeneration. Certain shoot meristem specification genes, notably WUSCHEL (WUS) and CLAVATA3, were significantly downregulated in the arr12 explants. Chromatin immunoprecipitation (ChIP) and transient activation assays demonstrated that ARR12 binds to the promoter of WUS. These observations indicate that during shoot regeneration, in vitro, ARR12 functions as a molecular link between cytokinin signaling and the expression of shoot meristem specification genes.