Microarray analysis of bast fibre producing tissues of Cannabis sativa identifies transcripts associated with conserved and specialised processes of secondary wall development
Microarray analysis of bast fibre producing tissues of Cannabis sativa identifies transcripts associated with conserved and specialised processes of secondary wall development
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DOI：
10.1071/fp07014
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发表时间：
2007-01-01
影响因子：
3
通讯作者：
Deyholos, Michael K.
中科院分区：
文献类型：
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作者：
De Pauw, Mary A.;Vidmar, John J.;Deyholos, Michael K.
大麻（Cannabis sativa L.）韧皮纤维分化的潜在机制在很大程度上是未知的。我们将cDNA微阵列与从茎轴三个不同位置提取的富含纤维的组织的RNA进行杂交。因此，我们鉴定出在韧皮纤维伸长或进行次生壁增厚的组织中富集的转录本。这些结果与细胞壁沉积的动态模式一致，该模式涉及大量不同的糖基转移酶和糖基水解酶的组织特异性表达，这些酶显然作用于含有半乳聚糖、甘露聚糖、木聚糖和葡聚糖以及棉子糖系列二糖的聚合物。假定的阿拉伯半乳聚糖蛋白和脂质转移蛋白是在各个茎段中高度富集的转录本之一，在发育的每个阶段表达不同的组合。我们还检测到油菜素内酯相关转录本、各种转运蛋白、多胺和苯丙烷类相关基因以及七种假定的转录因子的阶段特异性表达。最后，我们观察到许多具有未知生化功能的转录本的富集，其中一些先前已涉及杨树或棉花的纤维发育。这些数据共同补充和扩展了现有的韧皮纤维发育和次生壁沉积的生化模型，并突出了这些过程中未被表征但保守的成分。
The mechanisms underlying bast fibre differentiation in hemp ( Cannabis sativa L.) are largely unknown. We hybridised a cDNA microarray with RNA from fibre enriched tissues extracted at three different positions along the stem axis. Accordingly, we identified transcripts that were enriched in tissues in which phloem fibres were elongating or undergoing secondary wall thickening. These results were consistent with a dynamic pattern of cell wall deposition involving tissue specific expression of a large set of distinct glycosyltransferases and glycosylhydrolases apparently acting on polymers containing galactans, mannans, xylans, and glucans, as well as raffinose-series disaccharides. Putative arabinogalactan proteins and lipid transfer proteins were among the most highly enriched transcripts in various stem segments, with different complements of each expressed at each stage of development. We also detected stage-specific expression of brassinosteroid-related transcripts, various transporters, polyamine and phenylpropanoid related genes, and seven putative transcription factors. Finally, we observed enrichment of many transcripts with unknown biochemical function, some of which had been previously implicated in fibre development in poplar or cotton. Together these data complement and extend existing biochemical models of bast fibre development and secondary wall deposition and highlight uncharacterised, but conserved, components of these processes.