The maintenance of motility and the surface properties of epididymal spermatozoa from bull, rabbit and ram in homologous seminal and epididymal plasma.
The maintenance of motility and the surface properties of epididymal spermatozoa from bull, rabbit and ram in homologous seminal and epididymal plasma.
复制标题
同源精液和附睾浆中公牛、兔和公羊附睾精子的活力和表面特性的维持。
DOI：
--
复制
发表时间：
1979
期刊：
影响因子：
--
通讯作者：
G. C. Foster
中科院分区：
文献类型：
--
作者：
H. Dott;R. Harrison;G. C. Foster
将牛、兔和公羊的附睾精子分别置于含或不含血清白蛋白的生理盐水缓冲液中的同种附睾血浆或精浆中孵育。将精子直接稀释到培养基中或首先洗涤。未观察到洗涤对细胞对不同培养基的后续反应的影响。相当大比例的附睾精子群体在30 ℃的简单盐水培养基中存活（即继续表现出活力）长达22小时。最初，附睾血浆对公羊和公牛的精子活力有轻微的刺激作用，但对3种动物的精子存活率没有影响。精浆刺激运动显着公羊最初，但在所有3个物种精浆是不利于生存：在公羊，即使是15分钟的暴露于流体减少生存。血清白蛋白也刺激运动;它延迟，但不能阻止，精浆的有害影响，虽然它本身对生存没有影响。附睾血浆、精浆和血清白蛋白对附睾精子表面特性的影响，即凝集、粘玻璃和嗜酸性粒细胞增多。这些影响因物种而异，并且这些影响与对运动性和存活率的影响之间没有相关性。
Epididymal spermatozoa from bull, rabbit and ram were incubated in homologous epididymal plasma or seminal plasma in a buffered saline-based medium with or without serum albumin. The spermatozoa were either diluted directly into the medium or were washed first. No effect of washing was observed on the subsequent reaction of the cells to the different media. A considerable proportion of the populations of epididymal spermatozoa survived (i.e. continued to exhibit motility) for up to 22 h at 30 degrees C in the simple saline-based medium. Initially epididymal plasma had a slight stimulatory effect on sperm motility in ram and bull but it had no effect on sperm survival in any of the 3 species. Seminal plasma stimulated motility markedly in ram initially, but in all 3 species seminal plasma was detrimental to survival: in ram even a 15-min exposure to the fluid reduced survival. Serum albumin also stimulated motility; it delayed, but did not prevent, the detrimental effect of seminal plasma, although it had no effect itself on survival. The effects of epididymal plasma, seminal plasma and serum albumin on surface properties of epididymal spermatozoa, i.e. agglutination, sticking-to-glass and eosinophilia, were also noted. These varied between species and there was no correlation between these effects and the effects on motility and survival.