A multi-sensor system for measuring bovine embryo metabolism.
A multi-sensor system for measuring bovine embryo metabolism.
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DOI：
10.1016/j.bios.2018.09.071
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发表时间：
2019-02
影响因子：
12.6
通讯作者：
Yusra Obeidat;G. Catandi;E. Carnevale;A. Chicco;A. Demann;S. Field;Tom Chen
中科院分区：
文献类型：
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作者：
Yusra Obeidat;G. Catandi;E. Carnevale;A. Chicco;A. Demann;S. Field;Tom Chen
本文介绍了一种多传感器平台的开发，该平台能够在微室中同时测量溶解氧(DO)浓度、葡萄糖和乳酸浓度，以实时评估牛胚胎的代谢通量。用含DO、葡萄糖和乳酸三种感应器的微室，在呼吸缓冲液≤ 120 中测定单个卵母细胞或胚胎在不同发育阶段的代谢通量。在胚胎中，该传感器能够检测到从氧化磷酸化(OXPHOS)到糖酵解的代谢转变，这是通过减少氧气消耗和增加乳酸产生来证明的，寡霉素是线粒体三磷酸腺苷(ATP)合成的抑制剂。与OXPHOS相比，在胚胎中，随着乳酸/氧通量比率的逐渐增加，从桑椹胚到孵化囊胚的发育过程中，表现出对糖酵解的更多依赖，这与先前报道的孤立代谢评估一致。这些研究突出了代谢多传感器在牛胚胎有氧和无氧能量代谢的综合实时监测中的应用，在研究卵母细胞的代谢过程和早期胚胎发育方面具有潜在的应用前景。
This paper presents the development of a multi-sensor platform capable of simultaneous measurement of dissolved oxygen (DO) concentration, glucose and lactate concentrations in a micro-chamber for real-time evaluation of metabolic flux in bovine embryos. A micro-chamber containing all three sensors (DO, glucose, and lactate) was made to evaluate metabolic flux of single oocytes or embryos at different stages of development in ≤ 120 µL of respiration buffer. The ability of the sensor to detect a metabolic shift from oxidative phosphorylation (OXPHOS) to glycolysis was demonstrated in embryos by an ablation of oxygen consumption and an increase in lactate production following addition of oligomycin, an inhibitor of mitochondrial adenosine triphosphate (ATP) synthesis. An increased reliance upon glycolysis relative to OXPHOS was demonstrated in embryos as they developed from morula to hatched blastocysts by a progressive increase in the lactate/oxygen flux ratio, consistent with isolated metabolic assessments reported previously. These studies highlight the utility of a metabolic multi-sensor for integrative real-time monitoring of aerobic and anaerobic energy metabolism in bovine embryos, with potential applications in the study of metabolic processes in oocyte and early embryonic development.