B-cell exposure to self-antigen induces IL-10 producing B cells as well as IL-6-and TNF-α-producing B-cell subsets in healthy humans
B-cell exposure to self-antigen induces IL-10 producing B cells as well as IL-6-and TNF-α-producing B-cell subsets in healthy humans
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DOI：
10.1016/j.clim.2012.07.004
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发表时间：
2012-10-01
影响因子：
8.6
通讯作者：
Nielsen, Claus H.
中科院分区：
文献类型：
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作者：
Langkjaer, Anina;Kristensen, Birte;Nielsen, Claus H.
人B细胞在用有丝分裂原刺激后能够分泌IL-10，但它们在用自身抗原刺激后产生IL-10和调节T细胞应答的能力尚不清楚。我们将来自健康供体的甲状腺球蛋白脉冲的B细胞与自体T细胞共培养，观察IL-10和TGF-β以及TNF-α和IL-6的产生。脉冲与外来抗原，破伤风类毒素（TT），诱导了Th 1-反应与最小的IL-10的生产。甲状腺球蛋白脉冲后，1.10 +/- 0.50%的B细胞和1.00 +/- 0.20%的CD 4（+）T细胞产生IL-10，而TT脉冲后，B细胞为0.29 +/- 0.19%（P=0.01），CD 4（+）T细胞为0.13 +/- 0.15%（P=0.006）。甲状腺球蛋白刺激的IL-10分泌B细胞在CD 5（+）和CD 24（高）细胞中富集。而甲状腺球蛋白脉冲B细胞只诱导CD 4（+）T细胞的适度增殖，B细胞与TT脉冲诱导剧烈增殖。因此，B细胞在与T细胞的共培养物中介导自身抗原特异性IL-10、TNF-α和IL-6的产生，并积极地促进这些细胞因子的分泌。(c)2012 Elsevier Inc. All rights reserved.
Human B cells are able to secrete IL-10 after stimulation with mitogens, but their ability to produce IL-10 and regulate T-cell responses after stimulation with self-antigens is unclear. We co-cultured thyroglobulin-pulsed B cells from healthy donors with autologous T cells and observed production of IL-10 and TGF-beta, in addition to TNF-alpha and IL-6. Pulsing with foreign antigen, tetanus toxoid (TT), induced a Th1-response with minimal IL-10 production. After thyroglobulin-pulsing, 1.10 +/- 0.50% of B cells and 1.00 +/- 0.20% of CD4(+) T cells produced IL-10, compared to 0.29 +/- 0.19% of B cells (P=0.01) and 0.13 +/- 0.15% of CD4(+) T cells (P=0.006) following TT-pulsing. Thyroglobulin-stimulated, IL-10-secreting B cells were enriched within CD5(+) and CD24(high) cells. While thyroglobulin-pulsed B cells induced only modest proliferation of CD4(+) T cells, B cells pulsed with TT induced vigorous proliferation. Thus, B cells mediate self-antigen-specific IL-10, TNF-alpha and IL-6 production in co-cultures with T cells and contribute actively to these cytokine secretions. (c) 2012 Elsevier Inc. All rights reserved.