THE EFFECT OF INTERLEUKIN-1, INTERLEUKIN-6 AND ITS INTERRELATIONSHIP ON THE SYNTHESIS OF SERUM AMYLOID A-REACTIVE AND C-REACTIVE PROTEIN IN PRIMARY CULTURES OF ADULT HUMAN HEPATOCYTES
THE EFFECT OF INTERLEUKIN-1, INTERLEUKIN-6 AND ITS INTERRELATIONSHIP ON THE SYNTHESIS OF SERUM AMYLOID A-REACTIVE AND C-REACTIVE PROTEIN IN PRIMARY CULTURES OF ADULT HUMAN HEPATOCYTES
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DOI：
10.1016/s0006-291x(88)81056-8
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发表时间：
1988-08-30
影响因子：
3.1
通讯作者：
YAP, SH
中科院分区：
文献类型：
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作者：
MOSHAGE, HJ;ROELOFS, HMJ;YAP, SH
在急性期反应期间，C-反应蛋白和血清淀粉样蛋白A的合成增加。为了研究这些蛋白质的合成增强是否是由于巨噬细胞和单核细胞产生的炎性介质如白细胞介素-1（IL-1）和白细胞介素-6（IL-6）的刺激作用，在1 μ M地塞米松存在下，将成人肝细胞的原代培养物暴露于重组（r）IL-1、rIL-6或rIL-1和单特异性抗rIL-6抗体。结果表明，rIL-1和rIL-6都刺激肝脏合成C-反应蛋白和血清淀粉样蛋白A，然而，单特异性抗rIL-6抗体减少rIL-1对这些蛋白质的合成的刺激作用。这些结果表明，IL-6在刺激人肝细胞合成血清淀粉样蛋白A和C反应蛋白中起关键作用。
During the acute phase response, synthesis of C-reactive protein and serum amyloid A is increased. To investigate whether the enhanced synthesis of these proteins are due to stimulatory effect of inflammatory mediators such as interleukin-1 (IL-1) and interleukin-6 (IL-6) produced by macrophages and monocytes, primary cultures of adult human hepatocytes were exposed to recombinant (r)IL-1, rIL-6 or rIL-1 and monospecific anti rIL-6 antibodies in the presence of 1 .mu.M dexamethasone. The findings indicate that rIL-1 and rIL-6 both stimulate the liver synthesis of C-reactive protein and serum amyloid A, however monospecific anti rIL-6 antibodies reduce the stimulatory effect of rIL-1 on the synthesis of these proteins. These findings suggest that IL-6 plays a key role in the stimulation of synthesis of serum amyloid A and C-reactive protein by the human liver cells.