Cyclophosphamide-induced Hepatotoxicity in Wistar Rats: The Modulatory Role of Gallic Acid as a Hepatoprotective and Chemopreventive Phytochemical.
Cyclophosphamide-induced Hepatotoxicity in Wistar Rats: The Modulatory Role of Gallic Acid as a Hepatoprotective and Chemopreventive Phytochemical.
复制标题
DOI：
10.4103/2008-7802.177898
复制
发表时间：
2016
影响因子：
2.1
通讯作者：
Saba AB
中科院分区：
文献类型：
--
作者：
Oyagbemi AA;Omobowale OT;Asenuga ER;Akinleye AS;Ogunsanwo RO;Saba AB
没食子酸（GA）是一种内源性植物酚，已知具有抗氧化、自由基清除能力、抗炎、抗癌和抗真菌特性。本研究的目的是评估GA对环磷酰胺（CPA）诱导的雄性Wistar大鼠肝毒性的保护作用。将60只大鼠分为6组，每组10只大鼠。第1组接受蒸馏水。第2组在第1天腹腔内单次给予200 mg/kg CPA。第3组和第4组在第1天腹腔内接受单剂量CPA（200 mg/kg），然后分别以60和120 mg/kg体重的GA处理14天。第5组和第6组大鼠分别接受60和120 mg/kg体重的GA 14天。口服GA。肝组织病理学检查显示，CPA可引起肝组织天冬氨酸转氨酶、脏器重量的显著升高（P < 0.05）。CPA还可诱导肝脏氧化应激，表现为MDA含量显著升高（P < 0.05），过氧化氢（H_2O_2）生成量显著升高（P < 0.05），亚硝酸盐水平显著升高（P <0.05），谷胱甘肽（GSH）过氧化物酶水平显著降低（P <0.05）。GA显著提高了GSH水平、过氧化氢酶活性和GSH-S-转移酶活性（P < 0.05），增强了抗氧化系统。综上所述，本研究的结果表明，GA对CPA诱导的肝毒性具有保护作用。
Gallic acid (GA) is an endogenous plant phenol known to have antioxidant, free radical scavenging ability, anti-inflammatory, anti-cancer, and anti-fungal properties. The aim of this study was to assess the protective effect of GA on cyclophosphamide (CPA)-induced hepatotoxicity in male Wistar rats. Sixty rats were grouped into six groups of 10 rats per group. Group 1 received distilled water. Group 2 received CPA at 200 mg/kg single dose intraperitoneally on day 1. Groups 3 and 4 received a single dose of CPA (200 mg/kg) intraperitoneally on day 1 and then were treated with GA at 60 and 120 mg/kg body weight for 14 days, respectively. Rats in Groups 5 and 6 only received GA at 60 and 120 mg/kg body weight for 14 days, respectively. GA was administered orally. CPA induced hepatic damage as indicated by significant elevation (P < 0.05) in aspartate aminotransferase, organ weight, and evidence by the histological study. CPA also induced hepatic oxidative stress as indicated by significant elevation (P < 0.05) in malondialdehyde content, hydrogen peroxide (H2O2) generation, nitrite level, and the level of glutathione (GSH) peroxidase crashed in the CPA-treated group. GA enhanced the antioxidant defense system as indicated by significant elevation (P < 0.05) in GSH level, catalase activity, and GSH-S-transferase activity. Taken together, the result of this present study shows that GA has a protective effect on CPA-induced hepatotoxicity.