The effect of amiloride on hormonal regulation of amino acid transport in isolated and cultured adult rat hepatocytes.
The effect of amiloride on hormonal regulation of amino acid transport in isolated and cultured adult rat hepatocytes.
复制标题
阿米洛利对分离和培养的成年大鼠肝细胞中氨基酸转运的激素调节的影响。
DOI：
10.1016/0005-2736(81)90140-1
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Freychet,P
中科院分区：
文献类型：
--
作者：
Fehlmann,M;Samson,M;Koch,KS;Leffert,HL;Freychet,P
胰岛素和胰高血糖素刺激离体大鼠肝细胞的氨基酸转运。Amiloride是一种特异性Na+内流抑制剂，通过防止高亲和力转运成分的出现，完全抑制激素（胰高血糖素或胰岛素）对α-氨基异丁酸内流的刺激。该药还抑制[14C]缬氨酸并入肝细胞蛋白。抑制蛋白质合成的阿米洛利的半最大浓度与抑制激素刺激的氨基酸运输所需的半最大浓度相似(约为。0.1毫米)。在原代培养的大鼠肝细胞中，阿米洛利明显抑制胰高血糖素或胰高血糖素、胰岛素和表皮生长因子混合对α-氨基异丁酸转运的刺激。这些结果表明，阿米洛利通过阻止高亲和力转运蛋白的合成来抑制激素对肝细胞氨基酸转运的刺激。他们还认为，激素对肝细胞氨基酸运输的刺激至少部分依赖于Na+内流。
Insulin and glucagon stimulate amino acid transport in isolated rat hepatocytes. Amiloride, a specific Na+-influx inhibitor, completely inhibited the hormonal (glucagon or insulin) stimulation of α-aminoisobutyric acid influx by preventing the emergence of a high-affinity transport component.The drug also inhibited [14C]valine incorporation into hepatocyte protein. The half-maximal concentration of amiloride for inhibition of protein synthesis was similar to that required for inhibition of hormone-stimulated amino acid transport (approx. 0.1 mM). In primary cultured rat hepatocytes, amiloride markedly depressed the stimulation of α-aminoisobutyric acid transport by glucagon, or a mixture of glucagon, insulin and epidermal growth factor.These results suggest that amiloride inhibits the hormonal stimulation of hepatocyte amino acid transport by preventing the synthesis of high-affinity transport proteins. They also suggest that the hormonal stimulation of hepatocyte amino acid transport is dependent, at least partly, on Na+influx.