Intravital Microscopy and Thrombus Induction in the Earlobe of a Hairless Mouse
Intravital Microscopy and Thrombus Induction in the Earlobe of a Hairless Mouse
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DOI：
10.3791/55174
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发表时间：
2017-04-01
影响因子：
1.2
通讯作者：
Vollmar, Brigitte
中科院分区：
文献类型：
--
作者：
Stueder, Daniel;Grambow, Eberhard;Vollmar, Brigitte
血管疾病的血栓并发症是工业国家发病率和死亡率的主要原因之一。由于血栓形成过程中细胞和非细胞血液成分之间的复杂相互作用，对血栓形成的生理学和病理生理学的可靠研究只能在体内进行。因此，本文提出了一种无毛小鼠的耳模型，并着重于体内微循环、血栓形成和血栓演变的分析。通过使用活体荧光显微镜和静脉(IV)应用各自的荧光染料，可以很容易地对耳廓微循环进行重复分析，而不需要手术准备。此外，该模型还可用于不同问题的体内研究，包括伤口愈合、再灌注损伤或血管生成。综上所述，无毛小鼠的耳是在体研究生理或病理生理条件下皮肤微循环以及评价其对不同全身或局部治疗反应的理想模型。
Thrombotic complications of vascular diseases are one leading cause of morbidity and mortality in industrial nations. Due to the complex interactions between cellular and non-cellular blood components during thrombus formation, reliable studies of the physiology and pathophysiology of thrombosis can only be performed in vivo. Therefore, this article presents an ear model in hairless mice and focuses on the in vivo analysis of microcirculation, thrombus formation, and thrombus evolution. By using intravital fluorescence microscopy and the intravenous (iv) application of the respective fluorescent dyes, a repetitive analysis of microcirculation in the auricle can easily be performed, without the need for surgical preparation. Furthermore, this model can be adapted for in vivo studies of different issues, including wound healing, reperfusion injury, or angiogenesis. In summary, the ear of hairless mice is an ideal model for the in vivo study of skin microcirculation in physiological or pathophysiological conditions and for the evaluation of its reaction to different systemic or topical treatments.