Transcriptome-wide sequencing provides insights into geocarpy in peanut (Arachis hypogaea L.)
Transcriptome-wide sequencing provides insights into geocarpy in peanut (Arachis hypogaea L.)
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全转录组测序提供了对花生 (Arachishypogaea L.) 果果性的深入了解
DOI：
10.1111/pbi.12487
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发表时间：
2016-05-01
影响因子：
13.8
通讯作者：
Yu, Shanlin
中科院分区：
文献类型：
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作者：
Chen, Xiaoping;Yang, Qingli;Yu, Shanlin
花生的一个特征是地下结实，即地下结实，在这一过程中，雌蕊(peg‘)是一种专门的器官，在受精后从向上生长的习性转变为向下生长，驱动发育中的豆荚进入土壤中，以便随后在地下发育。作为理解这一现象的一步，我们探索了花生豆荚转录组在11个连续阶段的发育动力学。我们鉴定了110217个不同发育阶段的转录本，并沿着豆荚壁的发育梯度量化了它们的丰度。我们发现，大多数转录本在发育过程中存在差异表达，并发现了基因表达的时间程序，包括数百个转录因子。被认为是对特别恶劣的地下环境的适应，豆荚壁中上调和下调的基因集都得到了丰富，以应对一系列广泛的刺激，如重力、光线和地下环境因素。我们还鉴定了数百个与向重力性和光形态建成相关的转录本，这些转录本可能与地球结实有关。总体而言，这项研究形成了花生地果的转录基线，并提供了大量证据表明，花生地上和地下果实的转录调控是复杂的。
A characteristic feature of peanut is the subterranean fructification, geocarpy, in which the gynophore (peg'), a specialized organ that transitions from upward growth habit to downward outgrowth upon fertilization, drives the developing pod into the soil for subsequent development underground. As a step towards understanding this phenomenon, we explore the developmental dynamics of the peanut pod transcriptome at 11 successive stages. We identified 110217 transcripts across developmental stages and quantified their abundance along a pod developmental gradient in pod wall. We found that the majority of transcripts were differentially expressed along the developmental gradient as well as identified temporal programs of gene expression, including hundreds of transcription factors. Thought to be an adaptation to particularly harsh subterranean environments, both up- and down-regulated gene sets in pod wall were enriched for response to a broad array of stimuli, like gravity, light and subterranean environmental factors. We also identified hundreds of transcripts associated with gravitropism and photomorphogenesis, which may be involved in the geocarpy. Collectively, this study forms a transcriptional baseline for geocarpy in peanut as well as provides a considerable body of evidence that transcriptional regulation in peanut aerial and subterranean fruits is complex.