MicroRNA expression is differentially altered by xenobiotic drugs in different human cell lines.
MicroRNA expression is differentially altered by xenobiotic drugs in different human cell lines.
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DOI：
10.1002/bdd.764
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发表时间：
2011-09
影响因子：
2.1
通讯作者：
Yu, Ai-Ming
中科院分区：
文献类型：
--
作者：
Rodrigues, Alice C.;Li, Xin;Radecki, Laura;Pan, Yu-Zhuo;Winter, Jerrold C.;Huang, Min;Yu, Ai-Ming
一些非编码的microRNAs(miR或miRNA)已被证明可以调节药物代谢酶和转运蛋白的表达。异种生物药物诱导的酶和转运蛋白表达的变化可能与miRNA的表达变化有关。因此，本研究研究了19种异种药物(如地塞米松、长春新碱、白果内酯和可卡因)对MCF-7、Caco-2、SH-SY5Y和BE(2)-M17细胞系中10种miRNAs(miR-18a、-27a、-27b、-124a、-148a、-324-3p、-328、-451、-519c和-1291)表达的影响。我们的数据显示，miRNAs在人类细胞系中存在差异表达，并且miRNA表达的变化取决于药物和所研究的细胞类型。值得注意的是，白果内酯使Caco-2细胞miR-27A增加10倍，miR-148A减少2倍，而MCF-7细胞miR-27A无变化，miR-148A增加2倍。在BE(2)-M17和SH-SY5Y细胞中，精神活性药物(如可卡因、美沙酮和氟西汀)普遍下调神经元miR-124a的表达。地塞米松和长春花碱是药物代谢酶和转运蛋白的诱导剂，它们抑制了下调酶和转运蛋白的miR-27b、-148a和-451的表达。这些发现应该会增加对药物处置、多药耐药、药物-药物相互作用和神经可塑性的基因表达变化的理解。
Several noncoding microRNAs (miR or miRNA) have been shown to regulate the expression of drug-metabolizing enzymes and transporters. Xenobiotic drug-induced changes in enzyme and transporter expression may be associated with the alteration of miRNA expression. Therefore, this study investigated the impact of 19 xenobiotic drugs (e.g., dexamethasone, vinblastine, bilobalide and cocaine) on the expression of 10 miRNAs (miR-18a, -27a, -27b, -124a, -148a, -324-3p, -328, -451, -519c and -1291) in MCF-7, Caco-2, SH-SY5Y and BE(2)-M17 cell systems. Our data revealed that miRNAs were differentially expressed in human cell lines and the change in miRNA expression was dependent on the drug, as well as the type of cells investigated. Notably, treatment with bilobalide led to a 10-fold increase of miR-27a and a 2-fold decrease of miR-148a in Caco-2 cells, whereas no change of miR-27a and a 2-fold increase of miR-148a in MCF-7 cells. Neuronal miR-124a was generally down-regulated by psychoactive drugs (e.g., cocaine, methadone and fluoxetine) in BE(2)-M17 and SH-SY5Y cells. Dexamethasone and vinblastine, inducers of drug-metabolizing enzymes and transporters, suppressed the expression of miR-27b, -148a and -451 that down-regulate the enzymes and transporters. These findings should provide increased understanding of the altered gene expression underlying drug disposition, multidrug resistance, drug-drug interactions and neuroplasticity.
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影响因子：
29.4
作者：
Liu, Wan-Hsin;Yeh, Shiou-Hwei;Chen, Pei-Jer
通讯作者：
Chen, Pei-Jer
影响因子：
3
作者：
Harmsen, Stefan;Meijerman, I.;Febus, C. L.;Maas-Bakker, R. F.;Beijnen, J. H.;Schellens, J. H. M.
通讯作者：
Schellens, J. H. M.
影响因子：
2.4
作者：
Donnici L;Tiraboschi E;Tardito D;Musazzi L;Racagni G;Popoli M
通讯作者：
Popoli M
影响因子：
2.6
作者：
Biggs, Mary L.;Sorkin, Barbara C.;Fitzpatrick, Annette L.
通讯作者：
Fitzpatrick, Annette L.
DOI：
10.1073/pnas.0913517107
发表时间：
2010-01-26
影响因子：
11.1
作者：
Loven, Jakob;Zinin, Nikolay;Henriksson, Marie
通讯作者：
Henriksson, Marie