A possible coding for experience: ripple-like events and synaptic diversity.
A possible coding for experience: ripple-like events and synaptic diversity.
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一种可能的经验编码：类似涟漪的事件和突触多样性。
DOI：
10.1101/2019.12.30.891259
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Mitsushima D.
中科院分区：
文献类型：
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作者：
Ishikawa J;Tomokage T;Mitsushima D.
海马 CA1 对于维持包括人类在内的许多物种的经历过的情景记忆是必需的。为了监测处理的时间动态，我们记录了雄性大鼠在 10 分钟内经历以下四个事件之一的 CA1 神经元的多单元放电：约束压力、与雌性或雄性的社交互动或观察新物体。在体验之前，神经元大多表现出零星放电，并在习惯的笼子中出现一些同步（约 50 毫秒）的波纹状放电事件。经历发病后，约束或与其他老鼠的社交互动会间歇性地诱发自发高频放电（超级爆发），而物体观察诱发的事件不一致。体验开始后几分钟，CA1 神经元经常表现出波纹状放电，并伴有较少放电的静默期。涟漪状事件的数量取决于经历的事件并与超级爆发的总持续时间相关。以特定事件的方式体验明显多样化的单个波纹状事件的多个特征，在笼子中持续超过 30 分钟。离体膜片钳分析进一步揭示了体验促进的突触可塑性。与未暴露的对照组相比，经历雌性、雄性或束缚事件的动物表现出细胞依赖性的 AMPA 或 GABAA 受体介导的突触后电流增加，而与新物体接触仅增加 GABA 能电流。多维虚拟空间中的多元方差分析揭示了特定体验的超级爆发以及随后的涟漪状事件和突触可塑性，使我们假设这些因素负责创建特定体验的记忆。通过对多个 CA1 神经元中精心策划的波纹状放电和突触可塑性的深度学习，可以破译加密的体验。
The hippocampal CA1 is necessary to maintain experienced episodic memory in many species, including humans. To monitor the temporal dynamics of processing, we recorded multiple-unit firings of CA1 neurons in male rats experiencing one of four episodes for 10 min: restraint stress, social interaction with a female or male, or observation of a novel object. Before an experience, the neurons mostly exhibited sporadic firings with some synchronized (≈ 50 ms) ripple-like firing events in habituated home cage. After experience onset, restraint or social interaction with other rats induced spontaneous high-frequency firings (super bursts) intermittently, while object observation induced the events inconsistently. Minutes after experience initiation, CA1 neurons frequently exhibited ripple-like firings with less-firing silent periods. The number of ripple-like events depended on the episode experienced and correlated with the total duration of super bursts. Experience clearly diversified multiple features of individual ripple-like events in an episode-specific manner, sustained for more than 30 min in the home cage.Ex vivopatch clamp analysis further revealed experience-promoted synaptic plasticity. Compared with unexposed controls, animals experiencing the female, male, or restraint episodes showed cell-dependently increased AMPA- or GABAAreceptor– mediated postsynaptic currents, whereas contact with a novel object increased only GABAergic currents. Multivariate ANOVA in multi-dimensional virtual space revealed experience-specific super bursts with subsequent ripple-like events and synaptic plasticity, leading us to hypothesize that these factors are responsible for creating experience-specific memory. It is possible to decipher encrypted experience through the deep learning of the orchestrated ripple-like firings and synaptic plasticity in multiple CA1 neurons.