Identification of a second class I antigen controlled by the K end of the H-2 complex and its selective cellular expression.
Identification of a second class I antigen controlled by the K end of the H-2 complex and its selective cellular expression.
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由 H-2 复合物 K 端控制的第二类 I 抗原的鉴定及其选择性细胞表达。
DOI：
10.1073/pnas.80.5.1445
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发表时间：
1983
影响因子：
11.1
通讯作者：
Jones,PP
中科院分区：
文献类型：
--
作者：
Tryphonas,M;King,DP;Jones,PP
用H-2Kd和H-2Dd特异性单克隆抗体从[35 S]蛋氨酸标记的脾细胞中获得免疫沉淀物，用双向聚丙烯酰胺凝胶电泳分离。这些凝胶图谱的分析揭示了H-2d复合物K末端的另外产物的存在，在此称为H-2K ′。为了确定H-2K'是一种独特的蛋白质还是先前表征的H-2Kd组织相容性抗原的差异糖基化形式，检测了在衣霉素存在下标记的非糖基化分子。结果表明，H-2K和H-2K'具有不同的非糖基化多肽前体形式。完全糖基化和非糖基化分子之间的近似分子量差异也表明H-2K '上存在三个寡糖侧链，与H-2Kd的情况一样，而H-2Dd只有两个寡糖单元。同时观察了H-2K'的细胞表达。从正常脾细胞和在存在或不存在刀豆球蛋白A刺激的脾细胞的上清液的情况下培养的巯基乙酸盐诱导的粘附性腹膜渗出细胞免疫沉淀的H-2抗原的比较显示，H-2K'在粘附性腹膜细胞上不表达。这表明H-2K'以组织特异性方式表达，与经典的组织相容性抗原H-2K和H-2D不同。
Immunoprecipitates obtained from [35S]methionine-labeled spleen cells by using monoclonal antibodies specific for H-2Kd and H-2Dd have been separated by two-dimensional polyacrylamide gel electrophoresis. Analysis of these gel patterns revealed the presence of an additional product of the K end of the H-2d complex, designated here as H-2K'. To determine whether H-2K' is a unique protein or a differentially glycosylated form of the previously characterized H-2Kd histocompatibility antigen, nonglycosylated molecules labeled in the presence of tunicamycin were examined. The results showed that both H-2K and H-2K' have distinct nonglycosylated polypeptide precursor forms. The approximate molecular weight differences between the fully glycosylated and nonglycosylated molecules also indicated the presence of three oligosaccharide side chains on H-2K', as is the case with H-2Kd, whereas H-2Dd has only two oligosaccharide units. The cellular expression of H-2K' was also investigated. Comparison of H-2 antigens immunoprecipitated from normal spleen cells and from thioglycollate-induced adherent peritoneal exudate cells cultured in the presence or absence of supernatant fluids from concanavalin A-stimulated spleen cells revealed that H-2K' was not expressed on the adherent peritoneal cells. This indicates that H-2K' is expressed in a tissue-specific manner, unlike the classical histocompatibility antigens H-2K and H-2D.