Effect of Lonicerae Flos extracts on reflux esophagitis with antioxidant activity
Effect of Lonicerae Flos extracts on reflux esophagitis with antioxidant activity
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DOI：
10.3748/wjg.15.4799
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发表时间：
2009-10-14
影响因子：
4.3
通讯作者：
Roh, Seong-Soo
中科院分区：
文献类型：
--
作者：
Ku, Sae-Kwang;Seo, Bu-Il;Roh, Seong-Soo
目的：观察传统抗炎药金银花（LF）与著名的质子抗氧化剂α-生育酚相比，对幽门前胃结扎所致大鼠反流性食管炎（RE）的影响。方法：给大鼠口服三种不同剂量的金银花（500、250和125 mg/kg），每天一次，连续14 d。前胃结扎术。幽门和前胃结扎后九小时，观察食管和胃底的胃和食管病变区域、胃体积、胃酸和胃蛋白酶输出、抗氧化作用、食管脂质过氧化、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）、髓过氧化物酶和谷胱甘肽（GSH）水平以及胶原含量（灵活性标记）的变化组织病理学。将结果与已证实对RE效果的x-生育酚（一次口服，术前1小时，30 mg/kg）治疗组进行比较。结果：幽门和前胃结扎导致食管和胃粘膜大体病变面积明显增加，这与组织病理学变化相对应。此外，食管脂质过氧化增加，SOD、CAT和GSH自由基减少。然而，这些幽门和前胃结扎诱导的 RE 受到 500、250 和 125 mg/kg LF 提取物的剂量依赖性抑制，由 250 mg/kg RE 介导，显示出类似的 α-生育酚效应。结论：结果表明 LF 的抗氧化作用可以减轻 RE 的严重程度并预防食管粘膜损伤。损害，并验证其在食管反流病中的治疗作用（C）2009 The WIG Press 和 Baishideng 版权所有。
AIM: To observe the effects of traditional anti-inflammatory medicine Lonicerae Flos (LF) on rat reflux esophagitis (RE) induced by pylorus and forestomach ligation compared with the well-known proton antioxidant, alpha-tocopherol.METHODS: Rats were pretreated with three different dosages of LF (500, 250 and 125 mg/kg) orally, once a day for 14 d before pylorus and forestomach ligation. Nine hours after pylorus and forestomach ligation, changes to the stomach and esophagus lesion areas, gastric volumes, acid and pepsin outputs, antioxidant effects, esophageal lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), myeloperoxidase and glutathione (GSH) levels, and collagen contents (marker of flexibility) were observed on the esophageal and fundic histopathology. The results were compared with an (x-tocopherol (once orally, 1 h before operation, 30 mg/kg) treated group in which the effects on RE were already confirmed.RESULTS: Pylorus and forestomach ligations caused marked increases of gross esophageal and gastric mucosa lesion areas, which corresponded with histopathological changes. In addition, increases of esophageal lipid peroxidation, decreases of SOD, CAT, and GSH-free radical scavengers, increases of collagen were observed. However, these pylorus and forestomach ligation induced RE were dose-dependently inhibited by treatment of 500, 250 and 125 mg/kg of LF extract, mediated by antioxidant effects. RE at 250 mg/kg showed similar effects alpha-tocopherol.CONCLUSION: The results suggest that antioxidant effects of LF could attenuate the severity of RE and prevent the esophageal mucosal damage, and validate its therapeutic use in esophageal reflux disease. (C) 2009 The WIG Press and Baishideng. All rights reserved.