A glucose biosensor based on direct electrochemistry of glucose oxidase immobilized on nitrogen-doped carbon nanotubes
A glucose biosensor based on direct electrochemistry of glucose oxidase immobilized on nitrogen-doped carbon nanotubes
复制标题
基于固定在氮掺杂碳纳米管上的葡萄糖氧化酶直接电化学的葡萄糖生物传感器
DOI：
10.1016/j.bios.2009.07.016
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发表时间：
2009-10-15
影响因子：
12.6
通讯作者：
Ju, Huangxian
中科院分区：
文献类型：
--
作者：
Deng, Shengyuan;Jian, Guoqiang;Ju, Huangxian
将葡萄糖氧化酶（GOx）固定在氮掺杂碳纳米管（CNx-MWNTs）修饰电极上，制备了一种新型的葡萄糖生物传感器。由于碳纳米管的双原子侧吸附作用，碳纳米管膜对O-2的还原具有良好的电催化活性。氮的掺杂加速了电子从电极表面向固定化GOx的转移，导致GOx的直接电化学。利用扫描电镜、接触角和电化学阻抗技术对该生物功能表面进行表征，结果表明该生物功能表面具有良好的生物相容性、良好的电子传导网络和较大的比表面积。固定化GOx的直接电子转移导致稳定的安培生物传感葡萄糖的线性范围为0.02至1.02 mM和0.01 mM（S/N = 3）的检测限。这些结果表明，碳纳米管是一种很好的候选材料，用于构建基于固定化酶的直接电化学的第三代酶生物传感器。(C)2009 Elsevier B. V.保留所有权利。
A novel biosensor for glucose was prepared by immobilizing glucose oxidase (GOx) on nitrogen-doped carbon nanotubes (CNx-MWNTs) modified electrode. The CNx-MWNTs membrane showed an excellent electrocatalytic activity toward the reduction of O-2 due to its diatomic side-on adsorption on CNx-MWNTs. The nitrogen doping accelerated the electron transfer from electrode surface to the immobilized GOx, leading to the direct electrochemistry of GOx. The biofunctional surface showed good biocompatibility, excellent electron-conductive network and large surface-to-volume ratio, which were characterized by scanning electron microscopy, contact angle and electrochemical impedance technique. The direct electron transfer of immobilized GOx led to stable amperometric biosensing for glucose with a linear range from 0.02 to 1.02 mM and a detection limit of 0.01 mM (S/N = 3). These results indicated that CNx-MWNTs are good candidate material for construction of the third-generation enzyme biosensors based on the direct electrochemistry of immobilized enzymes. (C) 2009 Elsevier B.V. All rights reserved.