Gap junctions regulate responses of tendon cells ex vivo to mechanical loading.
Gap junctions regulate responses of tendon cells ex vivo to mechanical loading.
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DOI：
10.1097/00003086-199910001-00034
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发表时间：
1999-10
影响因子：
4.2
通讯作者：
A. Banes;Paul S. Weinhold;Xi Yang;M. Tsuzaki;D. Bynum;Michael Bottlang;Thomas G. Brown
中科院分区：
文献类型：
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作者：
A. Banes;Paul S. Weinhold;Xi Yang;M. Tsuzaki;D. Bynum;Michael Bottlang;Thomas G. Brown
利用禽指屈肌腱体外负荷装置，研究细胞间通讯被阻断对脱氧核糖核酸和胶原合成的影响。从52日龄的白来窝鸡中趾切除指深屈肌腱，作为无负荷对照，或夹在位移控制组织加载装置的颌部，以1 Hz的0.65%伸长率机械加载3天，每天8小时，休息16小时。在最后16小时用3h -胸腺嘧啶对肌腱样品进行放射性标记以监测脱氧核糖核酸的合成或用3h -脯氨酸对新合成的胶原进行放射性标记。全禽屈肌腱的循环加载刺激了脱氧核糖核酸和胶原的合成，这可以被辛醇（一种可逆的间隙连接阻滞剂）阻断。来自人指屈肌腱的细胞被用来填充一个矩形的，三维的，多孔的，聚酯泡沫，可以在体外循环变形。总之，这些结果支持了肌腱细胞必须通过交流来维持生长和基质表达的假设，并且一个工程化的三维结构可以用来研究体外机械负荷的反应。
Avian digital flexor tendons were used with a device to apply load ex vivo to study the effects on deoxyribonucleic acid and collagen synthesis when cell to cell communication is blocked. Flexor digitorum profundus tendons from the middle toe of 52-day-old White Leghorn chickens were excised and used as nonloaded controls, or clamped in the jaws of a displacement controlled tissue loading device and mechanically loaded for 3 days at a nominal 0.65% elongation at 1 Hz for 8 hours per day with 16 hours rest. Tendon samples were radiolabeled during the last 16 hours with 3H-thymidine to monitor deoxyribonucleic acid synthesis or with 3H-proline to radiolabel newly synthesized collagen. Cyclic loading of whole avian flexor tendons stimulated deoxyribonucleic acid and collagen synthesis, which could be blocked with octanol, a reversible gap junction blocker. Cells from human digital flexor tendon were used to populate a rectangular, three-dimensional, porous, polyester foam that could be deformed cyclically in vitro. Together, these results support the hypothesis that tendon cells must communicate to sustain growth and matrix expression and that an engineered three-dimensional construct can be used to study responses to mechanical load in vitro.