The effects of autologous bone marrow mesenchymal stem cell arterial perfusion on vascular repair and angiogenesis in osteonecrosis of the femoral head in dogs
The effects of autologous bone marrow mesenchymal stem cell arterial perfusion on vascular repair and angiogenesis in osteonecrosis of the femoral head in dogs
复制标题
自体骨髓间充质干细胞动脉灌注对犬股骨头坏死血管修复和血管生成的影响
DOI：
10.1007/s00264-012-1674-7
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发表时间：
2012-12-01
影响因子：
2.7
通讯作者：
Tong, Peijian
中科院分区：
文献类型：
--
作者：
Jin, Hongting;Xia, Bingjiang;Tong, Peijian
目的观察骨髓间充质干细胞（MSCs）动脉灌注对股骨头骨坏死（ONFH）血管修复和新生血管的影响。方法12只健康成年雄性Beagle犬随机分为A组（对照组）和B组（MSCs动脉灌注组）。采用髋关节脱位和液氮法建立ONFH动物模型。同时获得MSCs，进行培养和增殖。三周后，所有动物进行动脉灌注。B组给予MSCs 1 ml (5 × 106-1 × 107/ml)， a组给予0.9%生理盐水，4周或8周处死。观察大鼠大动脉、血管内皮生长因子（VEGF）、VEGF mRNA表达及微血管密度（MVD）的变化。所有数据均采用SPSS13.0软件进行分析。结果数字减影血管造影（DSA）显示，治疗4周、8周后，B组股骨头主干动脉数量和内径均较A组有所改善（P< 0.05，P< 0.01）。组织学和免疫组化方面，治疗4、8周后VEGF、MVD表达均显著高于A组（P< 0.05，P< 0.01）。实时定量聚合酶链反应（RT-PCR）显示，治疗4、8周后，B组VEGF mRNA表达量显著高于A组（P< 0.05、P<0.01），治疗8周后，B组VEGF mRNA表达量显著高于A组（P<0.01）。结论smscs动脉灌注可促进股骨头血管修复和新生血管生成，从而改善股骨头的血供和修复。
PurposeThe purpose of this study was to observe the effects of marrow mesenchymal stem cell (MSCs) arterial perfusion on vascular repair and angiogenesis in osteonecrosis of the femoral head (ONFH).MethodsTwelve healthy male adult Beagle dogs were randomly divided into two groups: group A (the control group) and group B (the MSCs arterial perfusion group). ONFH animal models were established by hip dislocation and liquid nitrogen. At the same time, MSCs were obtained, cultured and proliferated. After three weeks, arterial perfusion was performed in all animals. Group B was given 1 ml MSCs (5 × 106–1 × 107/ml), while 0.9 % normal saline was used in group A. After four weeks or eight weeks, the dogs were put to death. The changes of main arteries, the expression of vascular endothelial growth factor (VEGF), VEGF mRNA and microvessel density (MVD) of ONFH were observed. All the data were analysed by SPSS13.0.ResultsIn digital subtraction angiography (DSA), after four or eight weeks of treatment, the quantity and diameter of the main arteries of the femoral head in group B were improved, compared to group A (P< 0.05,P< 0.01). Concerning histology and immunohistochemistry, after four or eight weeks of treatment, the expression of VEGF and MVD were significantly higher than that of group A (P< 0.05,P< 0.01). For real-time quantitative polymerase chain reaction (RT-PCR), after four or eight weeks of treatment, the expression of VEGF mRNA in group B was significantly higher than that of group A (P< 0.05,P< 0.01), and after eight weeks of treatment, the expression of VEGF mRNA were significantly higher than that of four-weeks treatment in group A (P<0.01).ConclusionsMSCs arterial perfusion can promote vascular repair and angiogenesis and then improve blood supply and repair of femoral head.