In vivo effects of partial electrothermal shrinkage on mechanical properties of the anterior cruciate ligament in rabbits
In vivo effects of partial electrothermal shrinkage on mechanical properties of the anterior cruciate ligament in rabbits
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DOI：
10.1016/j.clinbiomech.2007.07.002
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发表时间：
2007-11-01
影响因子：
1.8
通讯作者：
Tohyama, Harukazu
中科院分区：
文献类型：
--
作者：
Kondo, Eiji;Yasuda, Kazunori;Tohyama, Harukazu
背景。没有进行过研究来澄清这一点。射频治疗前交叉韧带病变的体内重塑。目的是确定射频收缩对前交叉韧带力学性能的体内影响。使用30只骨骼成熟的兔子。在每组中，射频能量设置在非消融水平应用于前交叉韧带的后外侧束双极射频发生器。所有动物分别于术后0、6和12周处死。每组10只标本中分别取7只和3只进行生物力学和组织学评价。收缩治疗后，术后膝关节前后移位和后外侧束长度明显减少，但这种影响在6周后消失。处理后的束的抗拉强度和切线模量在各时段均明显低于正常对照束。此外，12周时的拉伸强度和切线模量明显低于0周时。组织学检查显示，12周时，后外侧束中间可见肉芽样组织，有大量丰满的成纤维细胞和炎症细胞。提示射频治疗后缩短的前交叉韧带组织随着时间的推移逐渐拉长。射频收缩导致的前交叉韧带后外侧束的力学性能在体内不能恢复，但随着时间的推移会明显恶化。(C) 2007 Elsevier Ltd.版权所有。
Background. No studies have been conducted to clarify an it? vivo remodeling of the radio frequency-treated lesion of the anterior cruciate ligament. The purpose was to determine in vivo effects of radiofrequency shrinkage on mechanical properties of the anterior cruciate ligament.Methods. Thirty skeletally mature rabbits were used. In each group, radiofrequency energy set at non-ablative levels was applied to the posterolateral bundle of the anterior cruciate ligament with a bipolar radioftequency generator. All animals were sacrificed at 0, 6, and 12 weeks after surgery, respectively. In each group, 7 and 3 out of the 10 specimens were used for biomechanical and histological evaluations.Findings. After shrinkage treatment, the anterior-posterior translation of the knee and the length of the posterolateral bundle were significantly reduced immediately after surgery, but that this effect disappeared at 6 weeks. The tensile strength and the tangent modulus of the treated bundle were significantly lower than that of the normal control bundle at each period. In addition, the tensile strength and the tangent modulus measured at 12 weeks were significantly lower than that at 0 week. Histological examination showed granulationlike tissues with numerous plump fibroblasts and inflammatory cells were dominantly found in the midsubstance of the posterolateral bundle at 12 weeks.Interpretations. This result suggested that the anterior cruciate ligament tissue shortened with the radioftequency treatment is elongated gradually over time. The mechanical properties of the posterolateral bundle of the anterior cruciate ligament reduced by the radiofrequency shrinkage are not restored in vivo, but significantly deteriorated with time. (C) 2007 Elsevier Ltd. All rights reserved.