Use of PCR analyses to define the distribution of Ralstonia species recovered from patients with cystic fibrosis
Use of PCR analyses to define the distribution of Ralstonia species recovered from patients with cystic fibrosis
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DOI：
10.1128/jcm.43.7.3463-3466.2005
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发表时间：
2005-07-01
影响因子：
9.4
通讯作者：
LiPuma, JJ
中科院分区：
文献类型：
--
作者：
Coenye, T;Spilker, T;LiPuma, JJ
两种新的PCR检测方法（罗尔斯通氏菌属和罗尔斯通氏菌属），以及以前发表的PCR检测方法，用于评估从111例囊性纤维化患者中回收的罗尔斯通氏菌分离株。解甘露醇罗尔斯通氏菌（Ralstonia mannitolilytica）占分离菌株的46%;皮氏罗尔斯通氏菌（Ralstonia pickettii）占18%。还鉴别了先前未从人体样本中回收的Ralstonia basilensis和Ralstonia metallidurans。
Two new PCR assays (for Ralstonia species and Ralstonia respiraculi), together with previously published PCR assays, were used to assess Ralstonia isolates recovered from 111 cystic fibrosis patients. Ralstonia mannitolilytica accounted for 46% of isolates, while R. respiraculi and Ralstonia pickettii accounted for 19% and 18%, respectively. Ralstonia basilensis and Ralstonia metallidurans, species not previously recovered from human samples, were also identified.