Mutations involving the endogenous ecotropic murine leukemia virus in primary mammary carcinomas of BALB/c mice.
Mutations involving the endogenous ecotropic murine leukemia virus in primary mammary carcinomas of BALB/c mice.
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BALB/c 小鼠原发性乳腺癌中涉及内源性亲嗜性鼠白血病病毒的突变。
DOI：
10.1016/0304-3835(95)04060-9
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发表时间：
1996
期刊：
影响因子：
9.7
通讯作者：
Asch,BB
中科院分区：
文献类型：
--
作者：
Natoli,F;Crowley,MR;Asch,HL;Stoler,DL;Asch,BB
内源性小鼠白血病病毒相关因子（MLVEs）在BALB/c小鼠原发性乳腺癌中经常过表达。因此，我们寻找与MLVE相关的突变，并在激素和化学诱导的乳腺肿瘤中发现了生态性MLVE的扩增序列。限制性片段长度多态性（RFLP）分析显示，在这些肿瘤的基因组中，DNA重排与1-10个或更多的生态性MLVE新拷贝一致。这是小鼠乳腺癌中除小鼠乳腺肿瘤病毒外涉及内源性逆转录病毒突变的第一个证据。
Endogenous murine leukemia virus-related elements (MLVEs) are often overexpressed in primary mammary carcinomas of BALB/c mice. We therefore searched for mutations associated with MLVEs and found amplified sequences of the ecotropic MLVE in hormonally and chemically induced mammary neoplasms. Restriction fragment length polymorphism (RFLP) analysis revealed DNA rearrangements consistent with 1–10 or more new copies of the ecotropic MLVE in the genome of these tumors. This is the first evidence of mutations involving an endogenous retrovirus other than mouse mammary tumor virus in mouse mammary carcinomas.