Thyrotropin-releasing hormone and amphetamine produce different patterns of behavioral excitation in rats.
Thyrotropin-releasing hormone and amphetamine produce different patterns of behavioral excitation in rats.
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促甲状腺激素释放激素和安非他明在大鼠中产生不同的行为兴奋模式。
DOI：
10.1016/0014-2999(81)90294-6
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发表时间：
1981
影响因子：
5
通讯作者：
PrangeJr,AJ
中科院分区：
文献类型：
--
作者：
Ervin,GN;Schmitz,SA;Nemeroff,CB;PrangeJr,AJ
我们比较了腹腔注射促甲状腺激素释放激素(TRH，pGlu-His-Pro-NH2；10，20，30和60 mg/kg)和0.3和2 mg/kg d-苯丙胺(分别为低安非他明和中安非他明)产生的觉醒和多动，方法是用行为抽样和计分的方法测量离散行为项目的出现。为了尽量减少影响活动的外部变量，大鼠被单独关在隔离的观察室内，并在习服2.5h后的白天进行测试。在此条件下，赋形剂(0.9%氯化钠)处理的大鼠处于非活动状态，在注射后一小时内采集的所有时间样本中，有80%的时间处于休息或睡眠状态。TRH和AMPH都有。从睡眠中产生显著的唤醒，但在所有测试剂量下，TRH产生的唤醒比中等剂量的安非他明要少。以及一种行为反应模式，既不同于低AMPH也不同于中等AMPH。适中的安眠药。在向前运动和站立方面有显著增加，但安培值较低。而TRH并非如此。TRH和Low Amph都是。增加美容(也许只是通过增加清醒)，但TRH未能增加嗅觉，这是Amph.诱导的兴奋的一个基本特征。与Amph.不同的是，TRH会产生湿狗抖动、飞行员竖起、尾巴抬高和牙齿打颤。两个模块。阿姆。与对照组相比，TRH的活性显著增加，通过光细胞计数进行评估，尽管Amph。效果更强劲。IP后缺乏觉醒。注射促甲状腺激素(10IU/kg)或黑素细胞刺激素释放抑制因子(Pro-Leu-Gly-NH2；60 mg/kg)证明TRH诱导的觉醒既不是通过激活脑垂体-甲状腺轴介导的，也不是由三肽的非特异性效应介导的。
We compared the arousal and hyperactivity produced by intraperitoneal (i.p) injections of thyrotropin-releasing hormone (TRH, pGlu-His-Pro-NH2; 10, 20, 30 and 60 mg/kg) and 0.3 and 2 mg/kg d-amphetamine (low and moderate amph., respectively) by measuring the occurrence of discrete behavioral items with a behavioral sampling and scoring method. To minimize extraneous variables affecting activity, rats were caged singly inside isolated observation chambers and tested in the daytime after a 2.5 h period of habituation. Under these conditions, vehicle (0.9% NaCl)-treated rats were inactive and either rested or slept through 80% of all time samples taken in the hour after injection. Both TRH and amph. produces significant arousal from sleeping, but TRH, at all doses tested, produced less arousal than moderate amph. and a pattern of behavioral responses which differed from both low and moderate amph. Moderate amph. produced marked increases in forward locomotion and rearing, but low amph. and TRH did not. Both TRH and low amph. increased grooming (perhaps simply by increasing wakefulness), but TRH failed to increase sniffing, a cardinal feature of amph.-induced excitement. Unlike amph., TRH produced wet-dog shakes, piloerection, tail elevation and teeth chattering. Both mod. amph. and TRH significantly produced increased activity when compared to controls as assessed with photocell counts, though the amph. effect was more robust. The lack of arousal after i.p. injections of thyroid-stimulating hormone (10 I.U./kg) or melanocyte-stimulating hormone release-inhibiting factor (Pro-Leu-Gly-NH2; 60 mg/kg) is evidence that TRH-induced arousal is neither mediated by activation of pitupituitary-thyroid axis nor by a non-specific effect of tripeptides generally.