Potential probiotic Lactobacillus plantarum VSG3 improves the growth, immunity, and disease resistance of tropical freshwater fish, Labeo rohita
Potential probiotic Lactobacillus plantarum VSG3 improves the growth, immunity, and disease resistance of tropical freshwater fish, Labeo rohita
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DOI：
10.1016/j.fsi.2012.12.008
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发表时间：
2013-02-01
影响因子：
4.7
通讯作者：
Oviya, M.
中科院分区：
文献类型：
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作者：
Giri, Sib Sankar;Sukumaran, V.;Oviya, M.
本研究评价了不同剂量植物乳杆菌VSG3对Labeo Rohita幼鱼抗嗜水气单胞菌感染的生长性能、免疫力和抗病能力的影响。在饲料中分别添加0(对照组)、10(6)、10(8)、10(10)cfu g(-1)的L植物芽孢杆菌VSG3，饲喂平均体重60g的鱼类。在饲喂后30天和60天分别检测各项生长和免疫指标。在摄食60d后用嗜水气单胞菌攻击鱼类，并在感染后10d记录死亡率。结果表明，添加VSG3 60d对罗非鱼的特定生长率(SGR)和饲料利用率有显著影响(P&t；0.05)。饲料中添加植物乳杆菌VSG3显著提高了L罗希塔仔鱼的血清溶菌酶和替代补体途径活性、吞噬功能和呼吸爆发活性。在饲料中添加10(8)cfu g(-1)的VSG3组的超氧化物歧化酶(SOD)活性最高(P<；0.05)。试验组在喂养30天后血清IgM水平显著高于对照组，而在喂养60天后血清IgM水平则相反。此外，投喂含10(8)cfu g(-1)植物L VSG3的饲料的鱼攻击后的存活率显著高于(P<；0.05)77.7%。这些结果综合表明，在L罗希塔日粮中添加10(8)cfu g(-1)的植物乳酸菌VSG3对提高L罗希塔的生长、免疫力和抗病能力是最理想的。(C)2012爱思唯尔有限公司。保留所有权利。
The study evaluated the effects of dietary doses of Lactobacillus plantarum VSG3 on the growth performance, immunity, and disease resistance of Labeo rohita juveniles against Aeromonas hydrophila infection. Fish (mean body weight 60 g) were fed with diet containing 0 (control), 10(6), 10(8), 10(10) cfu g(-1) L plantarum VSG3 for 60 days. Various growth and immune parameters were examined at 30 and 60 days of post-feeding. Fish were challenged with A. hydrophila 60 days post-feeding and mortalities were recorded over 10 days post-infection. Results showed that administration of VSG3 for 60 days had significant effects (P < 0.05) on the specific growth rate (SGR) and feed utilization efficiency of L. rohita. Dietary administration of L. plantarum VSG3 significantly increased the serum lysozyme and alternative complement pathway (ACP) activities, phagocytosis and respiratory burst activity in L rohita throughout the experimental period. The highest superoxide dismutase (SOD) activity (P < 0.05) was observed in the fish group fed diet containing VSG3 at 10(8) cfu g(-1). The serum IgM levels were significantly higher in the experimental groups compared to the control group after 30 days of feeding; but, the result was opposite after 60 days of feeding. Further, fish fed the diet containing 10(8) cfu g(-1) L plantarum VSG3 had significantly higher (P < 0.05) post-challenge survival rate (77.7%). These results collectively suggest that dietary supplementation of L plantarum VSG3 at 10(8) cfu g(-1) to L rohita is optimal for enhancing the growth, immunity, and disease resistance against A. hydrophila infection. (C) 2012 Elsevier Ltd. All rights reserved.