ACTIVATION OF C-SRC NEURON-SPECIFIC SPLICING BY AN UNUSUAL RNA ELEMENT INVIVO AND INVITRO
ACTIVATION OF C-SRC NEURON-SPECIFIC SPLICING BY AN UNUSUAL RNA ELEMENT INVIVO AND INVITRO
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DOI：
10.1016/0092-8674(92)90291-j
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发表时间：
1992-05-29
期刊：
影响因子：
64.5
通讯作者：
BLACK, DL
中科院分区：
文献类型：
--
作者：
BLACK, DL
一个保守的正作用RNA序列被发现是小鼠c-src N1外显子的神经元特异性剪接所必需的。该序列位于N1和4外显子之间的内含子中，靠近N1供体位点。通常，只有外显子N1的神经特异性剪接需要这个序列。当N1下游的内含子缩短时，组成外显子4受体的剪接也依赖于激活序列。体外重建src的神经元和非神经元剪接模式。HeLa细胞提取剪接的外显子4至外显子3，跳过外显子N1。Weri-1视网膜母细胞瘤细胞提取拼接外显子4到外显子N1以及外显子3。两种剪接模式都依赖于激活序列。仅含有激活序列的123nt RNA特异性地抑制了src剪接的两种模式，表明与激活子结合的因子是其作用所必需的。
A conserved positive-acting RNA sequence was found to be required for the neuron-specific splicing of the mouse c-src N1 exon. The sequence lies in the intron between exons N1 and 4, close to the N1 donor site. Normally, only the neural-specific splicing of exon N1 required this sequence. When the intron downstream of N1 was shortened, splicing at the constitutive exon 4 acceptor also became dependent on the activating sequence. The neuronal and nonneuronal patterns of src splicing were reconstituted in vitro. HeLa cell extracts spliced exon 4 to exon 3, skipping exon N1. Weri-1 retinoblastoma cell extracts spliced exon 4 to exon N1 as well as to exon 3. Both patterns of splicing were dependent on the activating sequence. A 123 nt RNA containing just the activating sequence specifically inhibited both patterns of src splicing, indicating that factors bound to the activator were required for its effects.