Synergy between enzymes from Aspergillus involved in the degradation of plant cell wall polysaccharides
Synergy between enzymes from Aspergillus involved in the degradation of plant cell wall polysaccharides
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DOI：
10.1016/s0008-6215(00)00066-5
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发表时间：
2000-08-07
影响因子：
3.1
通讯作者：
Visser, J
中科院分区：
文献类型：
--
作者：
de Vries, RP;Kester, HCM;Visser, J
研究了几种主链裂解酶和辅助酶对小麦粉中的水不溶性戊聚糖和甜菜果胶两种植物细胞壁多糖的协同降解作用。在大多数测试的酶之间观察到协同作用，尽管并不总是达到相同的程度。内切木聚糖酶和β-木糖苷酶对木聚糖主链的降解受α-L-阿拉伯呋喃糖苷酶和阿拉伯木聚糖a阿拉伯呋喃水解酶的作用影响最大，分别导致木糖释放增加2.5倍和两倍。阿魏酸释放阿魏酸酯酶A和4-O-甲基葡萄糖醛酸释放α-葡萄糖醛酸苷酶在很大程度上依赖于木聚糖骨架的降解内切木聚糖酶，但也受到其他酶的影响。果胶毛状区域的主链的降解导致β-半乳糖苷酶和内切半乳聚糖酶释放半乳糖的两倍增加，但并没有显着影响阿拉伯呋喃糖苷酶和内切阿拉伯糖酶释放阿拉伯糖。阿魏酸酯酶A从甜菜果胶中释放阿魏酸的影响最强烈的存在下，其他辅助酶。(C)2000爱思唯尔科技有限公司版权所有。
Synergy in the degradation of two plant cell wall polysaccharides, water insoluble pentosan from wheat flour tan arabinoxylan) and sugar beet pectin, was studied using several main-chain cleaving and accessory enzymes. Synergy was observed between most enzymes tested, although not always to the same extent. Degradation of the xylan backbone by endo-xylanase and beta-xylosidase was influenced most strongly by the action of alpha-L-arabinofuranosidase and arabinoxylan a rabinofuranohydrolase resulting in a 2.5-fold and twofold increase in release of xylose, respectively. Ferulic acid release by feruloyl esterase A and 4-O-methyl glucuronic acid release by alpha-glucuronidase depended largely on the degradation of the xylan backbone by endo-xylanase but were also influenced by other enzymes. Degradation of the backbone of the pectin hairy regions resulted in a twofold increase in the release of galactose by beta-galactosidase and endo-galactanase but did not significantly influence the arabinose release by arabinofuranosidase and endo-arabinase. Ferulic acid release from sugar beet pectin by feruloyl esterase A was affected most strongly by the presence of other accessory enzymes. (C) 2000 Elsevier Science Ltd. All rights reserved.