Proteomic analysis of parasitized Plutella xylostella larvae plasma.
Proteomic analysis of parasitized Plutella xylostella larvae plasma.
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DOI：
10.1016/j.jinsphys.2008.06.010
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发表时间：
2008-08
影响因子：
2.2
通讯作者：
K. Song;M. Jung;Jai-Hoon Eum;I. Hwang;S. Han
中科院分区：
文献类型：
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作者：
K. Song;M. Jung;Jai-Hoon Eum;I. Hwang;S. Han
昆虫利用它们的先天免疫来保护自己免受外来入侵者的侵害，如微生物，线虫和寄生虫。菜蛾绒茧蜂（Cotesia plutellae）是一种寄生小菜蛾（Plutella xylostella）的内寄生蜂，它使用几种策略来攻击宿主的免疫系统，如注射病毒、毒液和被称为畸形细胞的浆膜衍生细胞。然而，与这些免疫缺陷系统相关的蛋白质组图谱尚未明确定义。在这项研究中，我们调查的蛋白质表达模式的差异，寄生小菜蛾幼虫，以期确定寄生特异性因素。采用双向聚丙烯酰胺凝胶电泳技术，对寄生后每48 h的寄主血浆蛋白质进行分析。plutellae。共检测到350个蛋白质点，其中每48 h约有50个蛋白质点在小菜蛾幼虫体内差异表达。通过四极杆飞行时间串联质谱和序列同源性分析，总共鉴定了26个潜在的候选蛋白，包括小菜蛾丝氨酸蛋白酶抑制剂2（pxSerpin 2）、抑制性控制的肿瘤蛋白、信号转导组氨酸激酶、载脂蛋白-III和脂肪酸结合蛋白。这些蛋白质分为以下功能组：免疫、信号传导、脂质代谢、能量代谢、氨基酸/核苷酸代谢等。克隆了pxSerpin 2基因，并研究了其在寄生过程中的表达谱。pxSerpin 2的实时PCR分析显示mRNA水平和蛋白丰度之间的相关性较差。我们的研究结果清楚地表明，寄生虫特异性蛋白参与抑制宿主免疫反应。
Insects use their innate immunity to defend themselves against foreign invaders, such as microorganisms, nematodes and parasites. Cotesia plutellae, an endoparasitoid wasp that parasitizes the diamondback moth Plutella xylostella, uses several strategies to attack the host immune system, such as injection of viruses, venom, and serosal membrane-derived cells denoted teratocytes. However, the proteome profiles related to these immune deficiency systems have yet to be clearly defined. In this study, we investigate differences in protein expression patterns in parasitized P. xylostella larvae, with a view to identifying parasitism-specific factors. Using 2D polyacrylamide gel electrophoresis, proteins in the host plasma were assessed every 48h after parasitism by C. plutellae. A large number of protein spots (350 in total) were detected, and approximately 50 spots were differentially expressed in the parasitized P. xylostella larvae every 48h. In total, 26 potential candidates, including P. xylostella Serpin 2 (pxSerpin 2), translationally controlled tumor protein, signal transduction histidine kinase, apolipophorin-III, and fatty-acid binding protein were identified through quadrupole time-of-flight tandem mass spectrometry and sequence homology analysis. These proteins were classified into the following functional groups: immunity, signaling, lipid metabolism, energy metabolism, amino acid/nucleotide metabolism, and others. The pxSerpin 2 gene was cloned, and its expression profile investigated during the course of parasitism. Real-time PCR analysis of pxSerpin 2 revealed a poor correlation between the mRNA level and protein abundance. Our results clearly suggest that parasitism-specific proteins participate in suppression of the host immune response.