REARRANGEMENTS OF SYNAPTIC CONNECTIONS IN VISUAL-CORTEX REVEALED BY LASER PHOTOSTIMULATION
REARRANGEMENTS OF SYNAPTIC CONNECTIONS IN VISUAL-CORTEX REVEALED BY LASER PHOTOSTIMULATION
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DOI：
10.1126/science.7912852
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发表时间：
1994-07-08
期刊：
影响因子：
56.9
通讯作者：
KATZ, LC
中科院分区：
文献类型：
--
作者：
DALVA, MB;KATZ, LC
评估发育中的哺乳动物新皮质的突触连接模式主要依赖于解剖学研究。在此处描述的生理方法中，通过扫描激光光刺激确定发育中的雪貂视觉皮层切片中的突触连接模式。来自靠近神经元细胞体的位点的皮质第 2 层和第 3 层锥体细胞的功能性突触输入出现在睁眼前至少 2 周，即在形成远程水平连接之前。在接下来的10天的发展中出现了广泛的远程水平连接。本地连接数在睁眼时达到峰值；这些连接的数量随后下降到成人的水平，而长距离连接的特异性则增加。因此，局部连接对第 2 层和第 3 层神经元活动的相对影响随着皮层的成熟而下降，而较长距离连接的影响则大幅增加。
Assessing patterns of synaptic connections in the developing mammalian neocortex has relied primarily on anatomical studies. In a physiological approach described here, the patterns of synaptic connections in slices of developing ferret visual cortex were determined with scanning laser photostimulation. Functional synaptic inputs to pyramidal cells in cortical layers 2 and 3 originating from sites close to the neuronal cell body appeared at least 2 weeks before eye opening, prior to the formation of long-range horizontal connections. Extensive long-range horizontal connections appeared in the next 10 days of development. The number of local connections peaked at the time of eye opening; the number of these connections subsequently declined to the level found in the adult while the specificity of long-distance connections increased. Thus, the relative influence of local connections on the activity of layer 2 and layer 3 neurons declines as the cortex matures while the influence of longer range connections increases substantially.