Influence of Stream Bottom Substrate on Retention and Transport of Vertebrate Environmental DNA
Influence of Stream Bottom Substrate on Retention and Transport of Vertebrate Environmental DNA
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DOI：
10.1021/acs.est.6b01761
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发表时间：
2016-08-16
影响因子：
11.4
通讯作者：
Tank, Jennifer L.
中科院分区：
文献类型：
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作者：
Jerde, Christopher L.;Olds, Brett P.;Tank, Jennifer L.
虽然环境DNA（eDNA）现在经常被用来检测稀有和难以捉摸的物种，但在lotic环境中的检测需要注意：被检测到的物种可能距离采样点上游有一段距离。在这里，我们进行了一系列的自然流实验，以测试新的数字液滴PCR（ddPCR）的灵敏度，以检测低浓度的eDNA在一个lotic系统，测量eDNA的停留时间相比，一个保守的示踪剂，我们在这个系统中的eDNA的运输模型。我们发现，虽然ddPCR提高了我们的检测灵敏度，但eDNA的停留时间和运输并不遵循与保守示踪剂相同的动力学，并且需要一个更随机的框架来建模eDNA运输。没有证据表明由于底物类型的差异，eDNA的运输。相对大量的无法解释的变化，在eDNA运输揭示了需要揭示的机制和过程，通过该机制和过程，eDNA被运送到下游，导致物种检测，特别是当推论是在自然系统中，eDNA被用于保护管理。
While environmental DNA (eDNA) is now being regularly used to detect rare and elusive species, detection in lotic environments comes with a caveat: The species being detected is likely some distance upstream from the point of sampling. Here, we conduct a series of seminatural stream experiments to test the sensitivity of new digital droplet PCR (ddPCR) to detect low concentrations of eDNA in a lotic system, measure the residence time of eDNA compared to a conservative tracer, and we model the transport of eDNA in this system. We found that while ddPCR improves our sensitivity of detection, the residence time and transport of eDNA does not follow the same dynamics as the conservative tracer and necessitates a more stochastic framework for modeling eDNA transport. There was no evidence for differences in the transport of eDNA due to substrate type. The relatively large amount of unexplained variability in eDNA transport reveals the need for uncovering mechanisms and processes by which eDNA is transported downstream leading to species detections, particularly when inferences are to be made in natural systems where eDNA is being used for conservation management.