Differences in osteogenic differentiation of adipose-derived stromal cells from murine, canine, and human sources in vitro and in vivo.
Differences in osteogenic differentiation of adipose-derived stromal cells from murine, canine, and human sources in vitro and in vivo.
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DOI：
10.1097/prs.0b013e31821e6e49
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发表时间：
2011-08
影响因子：
3.6
通讯作者：
Longaker MT
中科院分区：
文献类型：
--
作者：
Levi B;Nelson ER;Brown K;James AW;Xu D;Dunlevie R;Wu JC;Lee M;Wu B;Commons GW;Vistnes D;Longaker MT
鉴于脂肪源性基质细胞来源和研究的物种多样性，作者着手描述可能存在于不同物种之间的基本细胞生物学差异。简而言之，作者发现不同物种间脂肪源性基质细胞的增殖和成骨潜力存在显著差异。如前所述，脂肪来源的基质细胞来源于人、小鼠和犬。将视黄酸、胰岛素样生长因子-1和骨形态发生蛋白-2加入到培养基中;通过标准化测定评估增殖和成骨分化。体内方法包括在仿生支架上接种150，000个脂肪来源的基质细胞，并通过微计算机断层扫描和组织学分析愈合。脂肪来源的基质细胞从所有物种进行成骨分化的能力。犬脂肪源性基质细胞增殖性最强，而人脂肪源性基质细胞增殖性最强，而人脂肪源性基质细胞成骨性最强（p < 0.05）。然而，人类细胞对成骨培养基有最显著的成骨反应。视黄酸刺激小鼠和犬细胞成骨，但不刺激人脂肪基质细胞。胰岛素样生长因子-1增强了所有物种的成骨作用，尤其是在人和犬源性细胞中。来源于人、小鼠和犬的脂肪源性基质细胞都具有成骨分化的能力。犬脂肪来源的基质细胞似乎是最增殖的，而人脂肪来源的基质细胞似乎是最成骨的。不同的细胞因子和化学物质可用于调节这种成骨反应。这些结果是有希望的，试图优化组织工程骨使用脂肪来源的基质细胞。
Given the diversity of species from which adipose-derived stromal cells are derived and studied, the authors set out to delineate the differences in the basic cell biology that may exist across species. Briefly, the authors found that significant differences exist with regard to proliferation and osteogenic potentials of adipose-derived stromal cells across species. Adipose-derived stromal cells were derived from human, mouse, and canine sources as previously described. Retinoic acid, insulin-like growth factor-1, and bone morphogenetic protein-2 were added to culture medium; proliferation and osteogenic differentiation were assessed by standardized assays. In vivo methods included seeding 150,000 adipose-derived stromal cells on a biomimetic scaffold and analyzing healing by micro–computed tomography and histology. Adipose-derived stromal cells from all species had the capability to undergo osteogenic differentiation. Canine adipose-derived stromal cells were the most proliferative, whereas human adipose-derived stromal cells were the most proliferative, whereas human adipose-derived stromal cells were the most osteogenic (p < 0.05). Human cells, however, had the most significant osteogenic response to osteogenic media. Retinoic acid stimulated osteogenesis in mouse and canine cells but not in human adipose-derived stromal cells. Insulin-like growth factor-1 enhanced osteogenesis across all species, most notably in human- and canine-derived cells. Adipose-derived stromal cells derived from human, mouse, and canine all have the capacity to undergo osteogenic differentiation. Canine adipose-derived stromal cells appear to be the most proliferative, whereas human adipose-derived stromal cells appear to be the most osteogenic. Different cytokines and chemicals can be used to modulate this osteogenic response. These results are promising as attempts are made to optimize tissue-engineered bone using adipose-derived stromal cells.