Bacteriorhodopsin conjugates as anchors for supported membranes.
Bacteriorhodopsin conjugates as anchors for supported membranes.
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细菌视紫红质缀合物作为支撑膜的锚定物。
DOI：
10.1021/bc034231h
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
GilchristJr,MLane
中科院分区：
文献类型：
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作者：
Sharma,ManojK;Jattani,Harsha;GilchristJr,MLane
随着新的应用领域的探索，支撑类脂双层膜的成熟度稳步提高。通常，系留的脂类被用来将脂类双层锚定在底物上。在这里，我们描述了一种新型的支撑脂质双层的锚定系统，它是基于生物素−PEG3400−细菌视紫红质偶联物。基于胺的偶联被用来构建聚合物偶联物，然后进行荧光团标记以实现共聚焦成像。基于细菌视紫红质的锚定系统被用来构建链霉亲和素包裹的微球的固体支撑囊泡。该方法为提高负载型脂质双层膜的稳定性提供了一条新的途径。
The sophistication of supported lipid bilayer membranes has increased steadily as new applications are being explored. In general, tethered lipids are used to anchor the lipid bilayer to the substrate. Here we describe a new type of anchoring system for supported lipid bilayers that is based on biotin−PEG3400−bacteriorhodopsin conjugates. Amine-based coupling was used to construct the polymer conjugates, followed by fluorophore labeling to enable confocal imaging. The bacteriorhodopsin-based anchoring system was used to construct solid-supported vesicles from streptavidin-coated microspheres. This method could provide a new route for the stability enhancement of supported lipid bilayer membrane assemblies.