Relationship between Polyamines and Anaerobic Respiration of Wheat Seedling Root under Water-Logging Stress
Relationship between Polyamines and Anaerobic Respiration of Wheat Seedling Root under Water-Logging Stress
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DOI：
10.1134/s1021443718060055
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发表时间：
2018-11-01
影响因子：
1.4
通讯作者：
Kurtenbach, R.
中科院分区：
文献类型：
--
作者：
Du, H. Y.;Liu, D. X.;Kurtenbach, R.
为阐明多胺与小麦无氧呼吸的关系，研究了多胺对小麦无氧呼吸的影响。以豫麦18号和扬麦9号两个小麦品种为材料，研究了淹水胁迫下小麦幼苗根系中多胺、乳酸和乙醇含量以及无氧呼吸酶活性的变化。淹水处理后第5天，亚精胺（Spd）和精胺（Spm）含量显著升高，丙酮酸脱羧酶（PDC）活性升高，但品种间差异不显著。扬麦9号的乙醇脱氢酶（ADH）活性和乙醇含量比豫麦18号显著提高，而豫麦18号的乳酸脱氢酶（LDH）活性和乳酸含量比扬麦9号显著提高。外源Spd和Spm处理促进了乙醇脱氢酶（ADH）活性、酒精含量以及Spd和Spm水平的提高。这同时抑制了淹水胁迫下豫麦18号LDH活性和乳酸含量的升高，减轻了胁迫对幼苗的伤害。外源抑制剂甲基甘氨酰双胍酰腙（MGBG）处理可抑制淹水胁迫下扬麦9号幼苗ADH活性、乙醇含量、Spd和Spm含量的升高，促进LDH活性和乳酸含量的升高，加重胁迫对幼苗的伤害。结果表明，在淹水胁迫下，Spd和Spm的增加可能通过促进ADH活性和乙醇含量的增加，抑制LDH活性和乳酸含量的增加，从而增强小麦幼苗对淹水胁迫的耐受性。
To elucidate the relationship between polyamines and anaerobic respiration of wheat (Triticum aestivum L.) seedling root under water-logging stress, the contents of polyamines (PAs), lactate and alcohol, and the activities of anaerobic respiration enzymes were investigated in seedling roots of two wheat cultivars, Yumai no. 18 and Yangmai no. 9. On the 5th day after water-logging treatment, spermidine (Spd) and spermine (Spm) contents increased significantly, pyruvate decarboxylase (PDC) activity increased and there was no difference between two cultivars. Alcohol dehydrogenase (ADH) activity and alcohol content in Yangmai no. 9 increased more markedly than Yumai no. 18, while lactate dehydrogenase (LDH) activity and the lactate content in the Yumai no. 18 increased more markedly than Yangmai no. 9. Treatments with exogenous Spd and Spm resulted in enhancing the increases in ADH activity, alcohol content, and the levels of Spd and Spm. This concomitantly inhibited the increases in LDH activity and lactate content in Yumai no. 18 under water-logging stress, alleviating stress-induced injury to the seedlings. Treatment with exogenous inhibitor methylglyoxyl-bis-guanylhydrazone (MGBG), resulted in reducing the increases in ADH activity, alcohol content, and Spd and Spm levels, promoting the increases in LDH activity and lactate content in Yangmai no. 9 under water-logging stress, and aggravating the stress-induced injury to the seedlings. The results suggested that under water-logging stress, increased Spd and Spm could facilitate the tolerance of wheat seedling to the stress by enhancing the increases in ADH activity and alcohol content, and inhibiting the increases in LDH activity and lactate content.