COMPLEMENT - SUBSTITUTION OF TERMINAL COMPONENT IN IMMUNE HEMOLYSIS BY 1,10-PHENANTHROLINE
COMPLEMENT - SUBSTITUTION OF TERMINAL COMPONENT IN IMMUNE HEMOLYSIS BY 1,10-PHENANTHROLINE
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DOI：
10.1126/science.157.3787.442
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发表时间：
1967-01-01
期刊：
影响因子：
56.9
通讯作者：
MULLEREB.HJ
中科院分区：
文献类型：
--
作者：
HADDING, U;MULLEREB.HJ
用螯合剂研究了金属离子在免疫细胞溶解最终步骤中的参与。 1,10-菲咯啉诱导致敏绵羊红细胞裂解，该红细胞与人补体的前八种成分发生反应。 1,10-菲咯啉引起的溶血类似于补体第九组分产生的裂解，依赖于细胞结合的第八组分和温度以及0.09 M乙二胺四乙酸的抑制。二价金属离子降低了1,10-菲咯啉的溶血能力，Fe++抑制了第九组分的活性。由于三价铁没有这样的作用，因此推测 1,10-菲咯啉和补体第九种成分的溶血活性是它们对 Fe++ 的亲和力的函数。
The participation of metal ions in the terminal step of immune cytolysis was investigated with chelating agents. 1,10-Phenanthroline induced lysis of sensitized sheep erythrocytes which had reacted with the first eight components of human complement. Hemolysis caused by 1,10-phenanthroline resembled lysis produced by the ninth component of complement in dependence on cell-bound eighth component and on temperature and in inhibition by 0.09 M ethylenediaminetetraacetic acid. Bivalent metal ions reduced the hemolytic capacity of 1,10-phenanthroline, and Fe++ inhibited the activity of the ninth component. Since trivalent iron had no such effects, it is postulated that the hemolytic activity of 1,10-phenanthroline and the ninth component of complement is a function of their affinity for Fe++.