Analysis and glycosyl composition of the exopolysaccharide isolated from the floc-forming wastewater bacterium Thauera sp MZ1T
Analysis and glycosyl composition of the exopolysaccharide isolated from the floc-forming wastewater bacterium Thauera sp MZ1T
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DOI：
10.1111/j.1462-2920.2004.00615.x
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发表时间：
2004-08-01
影响因子：
5.1
通讯作者：
Sayler, GS
中科院分区：
文献类型：
--
作者：
Allen, MS;Welch, KT;Sayler, GS
开发了一种新型微生物胞外多糖的可重复生产、分离和表征条件，该多糖被认为参与工业废水处理厂的瞬时粘性膨胀。从在含有琥珀酸盐的基本培养基上生长的Thauera sp.菌株MZ 1 T的无细胞培养上清液中提取胞外多糖。通过凝胶渗透色谱法发现纯化的聚合物大小约为260 kDa。糖醇乙酸酯和全-O-三甲基硅基甲基糖苷衍生物的GC-MS分析表明，胞外多糖由四种单糖组成：鼠李糖、半乳糖醛酸、N-乙酰葡萄糖胺和N-乙酰岩藻糖胺。葡萄糖也以低水平出现，最有可能来自共洗脱葡聚糖。FTIR和NMR光谱分析进一步揭示了聚合物上酯化组分基团的存在。这些结果代表了第一次发表的描述，从属Thauera的成员的多糖，并奠定了基础，更深入地了解潜在参与的因素zoogloeal集群的形成和粘性膨胀。
Conditions were developed for the reproducible production, isolation and characterization of a novel microbial extracellular polysaccharide believed to be involved in transient viscous bulking at an industrial wastewater treatment plant. The exopolysaccharide was extracted from cell-free culture supernatants of Thauera sp. strain MZ1T grown on a minimal medium with succinate. The purified polymer was found to be approximately 260 kDa in size by gel-permeation chromatography. The GC-MS analysis of the alditol acetate and per-O-trimethylsilyl methyl glycoside derivatives revealed that the exopolysaccharide was composed of four monosaccharides including: rhamnose, galacturonic acid, N-acetylglucosamine and N-acetylfucosamine. Glucose, which also appeared at low levels, is most likely from a co-eluting glucan. The FTIR and NMR spectroscopic analyses further revealed the presence of esterified component groups on the polymer. These results represent the first published description of a polysaccharide from a member of the genus Thauera, and lay the foundation for a deeper understanding of the factors potentially involved in zoogloeal cluster formation and viscous bulking.