Abscisic acid signaling and protein synthesis requirements for phosphoenolpyruvate carboxylase transcript induction in the common ice plant
Abscisic acid signaling and protein synthesis requirements for phosphoenolpyruvate carboxylase transcript induction in the common ice plant
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普通冰植物中磷酸烯醇丙酮酸羧化酶转录诱导的脱落酸信号传导和蛋白质合成要求
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
J. Cushman
中科院分区：
文献类型：
--
作者：
Tahar Taybi;J. Cushman
由水分亏缺、渗透或离子胁迫或脱落酸（阿坝）处理引起的景天科酸代谢诱导由钙依赖性信号传导途径介导。为了了解胁迫信号和阿坝之间的关系，以及由这些刺激激活的下游信号事件之间的关系，阿坝含量测定在离体的Mesembryocarcinum stac um叶片暴露于渗透胁迫（脱水或甘露醇），离子胁迫（NaCl）或外源阿坝处理。脱水和外源阿坝的应用导致阿坝积累的大幅增加，而甘露醇处理引起的阿坝积累只有一个小的增加。NaCl暴露未能诱导阿坝积累的显着变化，但导致CAM诱导Ppc 1，它编码的磷酸烯醇丙酮酸羧化酶（PEPC）的CAM特异性异构体的转录物的积累增加所测量的。离体叶片的阿坝生物合成抑制剂，氟立酮，预处理未能抑制Ppc 1转录积累胁迫诱导。综上所述，这些结果表明，应力诱导CAM基因的表达可能发生通过ABA依赖和ABA非依赖的信号通路。其他药理学研究表明磷酸肌醇在CAM诱导信号传导过程中的作用。预处理的离体叶片放线菌酮抑制Ppc 1的转录积累，在响应非生物胁迫和阿坝的应用表明，信号和/或转录激活事件，导致CAM基因表达的诱导依赖于蛋白质的合成。
Summary Crassulacean acid metabolism induction by water deficit, osmotic or ionic stress, or abscisic acid (ABA) treatment is mediated by a calcium-dependent signaling pathway. To understand the relationship between stress signals and ABA and between the downstream signaling events activated by these stimuli, ABA content was determined in detached Mesembryanthemum crystallinum leaves exposed to osmotic stress (dehydration or mannitol), ionic stress (NaCl) or treated with exogenous ABA. Dehydration and exogenous ABA application led to large increases in ABA accumulation, whereas mannitol treatment caused only a small increase in ABA accumulation. NaCl exposure failed to induce a significant change in ABA accumulation, yet resulted in CAM induction as measured by the increased accumulation of transcripts of Ppc1 , which encodes a CAM-specific isoform of phospho enol pyruvate carboxylase (PEPC). Pretreatment of detached leaves with the ABA biosynthesis inhibitor, Fluridone, failed to inhibit Ppc1 transcript accumulation following stress induction. Taken together, these results suggest that stress-induced CAM gene expression may occur via both ABA-dependent and ABA-independent signaling pathways. Additional pharmacological investigations suggest a role for phosphoinositides in the CAM-induction signaling process. Pre-treatment of detached leaves with cycloheximide inhibited Ppc1 transcript accumulation in response to abiotic stresses and ABA application demonstrating that signaling and/or transcriptional activation events leading to the induction of CAM gene expression are dependent upon protein synthesis.
影响因子：
56.9
作者：
Wu, Y;Kuzma, J;Chua, NH
通讯作者：
Chua, NH
DOI：
10.1016/0005-2736(89)90529-4
发表时间：
1989-02-13
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
GABEV, E;KASIANOWICZ, J;MCLAUGHLIN, S
通讯作者：
MCLAUGHLIN, S
影响因子：
7.4
作者：
DeWald, DB;Torabinejad, J;Hama, H
通讯作者：
Hama, H