Reproducibility of broth microdilution MICs for the novel siderophore cephalosporin, cefiderocol, determined using iron-depleted cation-adjusted Mueller-Hinton broth
Reproducibility of broth microdilution MICs for the novel siderophore cephalosporin, cefiderocol, determined using iron-depleted cation-adjusted Mueller-Hinton broth
复制标题
DOI：
10.1016/j.diagmicrobio.2019.03.003
复制
发表时间：
2019-08-01
影响因子：
2.9
通讯作者：
Sahm, Daniel F.
中科院分区：
文献类型：
--
作者：
Hackel, Meredith A.;Tsuji, Masakatsu;Sahm, Daniel F.
2017年，临床和实验室标准化研究所（CLSI）抗菌药物敏感性试验小组委员会批准使用Chelex（R）100树脂（Bio-Rad Laboratories，Hercules，CA）制备的缺铁阳离子调节Mueller-Hinton肉汤（ID-CAMHB）测定头孢地罗的MIC。本研究检查了19株革兰氏阴性杆菌临床分离株（使用3家生产商生产的CAMHB）生成的头孢地罗可MIC的重现性;在各生产商的ID-CAMHB中对19株分离株进行了10次重复检测。当按单个培养基批次进行分析时，对于19种供试分离株中的每一种，95%以上的MIC结果均在该模式的+/-1倍稀释范围内。其余5.0%的MIC结果在模式MIC的+/-2倍稀释范围内。对于合并的所有培养基批次，92.2%的MIC结果在每种分离株模式MIC的+/-1倍稀释范围内，99.8%在+/-2倍稀释范围内，100%在3倍稀释范围内。(C)2019年，任作家。爱思唯尔公司出版
In 2017, the Clinical and Laboratory Standards Institute (CLSI) Subcommittee on Antimicrobial Susceptibility Testing approved the use of iron-depleted cation-adjusted Mueller-Hinton broth (ID-CAMHB) prepared with Chelex (R) 100 resin (Bio-Rad Laboratories, Hercules, CA) to determine MICs for cefiderocol. The current study examined the reproducibility of cefiderocol MICs generated for 19 clinical isolates of Gram-negative bacilli, with CAMHB produced by three manufacturers; each of the 19 isolates was tested for 10 replicates in ID-CAMHB from each manufacturer. When analyzed by individual media lot, greater than 95% of MIC results were within +/- one doubling-dilution of the mode for each of the 19 isolates tested. The remaining 5.0% of MIC results were within +/- two doubling-dilutions of the modal MIC. For all media lots combined, 92.2% of MIC results were within +/- one doubling-dilution of the modal MIC for each isolate, 99.8% were within +/- two doubling-dilutions and 100% were within three doubling-dilutions. (C) 2019 The Authors. Published by Elsevier Inc.