Influence of engineered titania nanotubular surfaces on bone cells
Influence of engineered titania nanotubular surfaces on bone cells
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DOI：
10.1016/j.biomaterials.2007.03.020
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发表时间：
2007-07-01
期刊：
影响因子：
14
通讯作者：
Desai, Tejal A.
中科院分区：
文献类型：
--
作者：
Popat, Ketul C.;Leoni, Lara;Desai, Tejal A.
当前骨科生物材料研究的一个目标是设计诱导受控、引导和快速愈合的植入物。除了加速正常的伤口愈合现象外，这些植入物还应导致形成具有足够生物力学性能的特征界面层。然而，为了实现这些目标，需要更好地了解骨-材料界面的事件，以及开发促进骨整合的新材料和方法。使用阳极氧化，可以制造具有受控纳米结构的二氧化钛界面。这项研究证明了这些表面促进成骨细胞分化和基质产生的能力，并在体外增强短期和长期的骨整合。二氧化钛纳米管表面制作使用阳极氧化技术。从雄性刘易斯大鼠中分离骨髓基质细胞（MSC），并将其与对照表面一起沿着在这些表面上。细胞与这些表面的相互作用进行了研究，在他们的能力，粘附，增殖和分化。用来自不同培养物的细胞重复实验三次。所有结果均采用方差分析（ANOVA）进行分析。统计学显著性被认为是p < 0.05。此外，通过在雄性刘易斯大鼠皮下植入表面并在4周后进行组织学分析来评估体内生物相容性。我们的研究结果表明，纳米管二氧化钛表面提供了一个有利的骨细胞的生长和粘附的模板。在纳米管表面培养的细胞显示出更高的粘附，增殖，ALP活性和骨基质沉积相比，那些生长在平面钛表面。体内生物相容性结果表明，纳米管二氧化钛不会引起慢性炎症或纤维化。二氧化钛纳米结构的制造路线灵活且具有成本效益，能够在现有的非平面骨科植入物上实现所需的平台拓扑结构。(C)2007爱思唯尔有限公司保留所有权利。
A goal of current orthopedic biomaterials research is to design implants that induce controlled, guided, and rapid healing. In addition to acceleration of normal wound healing phenomena, these implants should result in the formation of a characteristic interfacial layer with adequate biomechanical properties. To achieve these goals, however, a better understanding of events at the bone-material interface is needed, as well as the development of new materials and approaches that promote osseointegration. Using anodization, titania interfaces can be fabricated with controlled nanoarchitecture. This study demonstrates the ability of these surfaces to promote ostcoblast differentiation and matrix production, and enhance short- and long-term osseointegration in vitro. Titania nanotubular surfaces were fabricated using an anodization technique. Marrow stromal cells (MSCs) were isolated from male Lewis rats and seeded on these surfaces along with control surfaces. The interaction of cells with these surfaces was investigated in terms of their ability to adhere, proliferate and differentiate on them. The experiments were repeated three times with cells from different cultures. All the results were analyzed using analysis of variance (ANOVA). Statistical significance was considered at p < 0.05. Furthermore, in vivo biocompatibility was assessed by implanting surfaces subcutaneously in male Lewis rat and performing histological analysis after 4 weeks. Our results indicate that the nanotubular titania surfaces provide a favorable template for the growth and maintainence of bone cells. The cells cultured on nanotubular surfaces showed higher adhesion, proliferation, ALP activity and bone matrix deposition compared to those grown on flat titanium surfaces. In vivo biocompatibility results suggest that nanotubular titania does not cause chronic inflammation or fibrosis. The fabrication routes of titania nano-architectures are flexible and cost-effective, enabling realization of desired platform topologies on existing non-planar orthopedic implants. (C) 2007 Elsevier Ltd. All rights reserved.