In situ probing of cholesterol in astrocytes at the single-cell level using laser desorption ionization mass spectrometric imaging with colloidal silver
In situ probing of cholesterol in astrocytes at the single-cell level using laser desorption ionization mass spectrometric imaging with colloidal silver
复制标题
DOI：
10.1002/rcm.4491
复制
发表时间：
2010-04-01
影响因子：
2
通讯作者：
Lee, Young Jin
中科院分区：
文献类型：
--
作者：
Perdian, D. C.;Cha, Sangwon;Lee, Young Jin
质谱成像已被用于定位单个星形胶质细胞，并在胶体银激光解吸电离（LDI）中获得单细胞水平的胆固醇种群。监测膜结合胆固醇的银离子加合物以检测单个细胞。在不同细胞密度下质谱和光学图像之间良好的相关性表明有能力进行胆固醇丰度的单细胞研究。LDI-MS离子信号与传统酶荧光测定结果一致，证实了定量的可行性。我们提出，这种方法可能是在细胞水平上研究化学种群的有效工具。John Wiley & Sons， Ltd出版于2010年C版。
Mass spectrometric imaging has been utilized to localize individual astrocytes and to obtain cholesterol populations at the single-cell level in laser desorption ionization (LDI) with colloidal silver. The silver ion adduct of membrane-bound cholesterol was monitored to detect individual cells. Good correlation between mass spectrometric and optical images at different cell densities indicates the ability to perform single-cell studies of cholesterol abundance. The feasibility of quantification is confirmed by the agreement between the LDI-MS ion signals and the results from a traditional enzymatic fluorometric assay. We propose that this approach could be an effective tool to study chemical populations at the cellular level. Published in (C) 2010 by John Wiley & Sons, Ltd.