Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor
Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor
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DOI：
10.1126/science.1081412
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发表时间：
2003-03-14
期刊：
影响因子：
56.9
通讯作者：
Mitchison, TJ
中科院分区：
文献类型：
--
作者：
Straight, AF;Cheung, A;Mitchison, TJ
在胞质分裂期间完成细胞分裂需要从微管细胞骨架到肌动蛋白细胞骨架和细胞膜的时间和空间调节的通信。我们确定了一种非肌肉肌球蛋白II的特异性抑制剂，blebbistatin，抑制卵裂沟的收缩，而不破坏有丝分裂或收缩环组装。使用blebbistatin和其他药物，我们表明，退出细胞周期的细胞动力学阶段取决于泛素介导的蛋白水解。来自微管的连续信号需要维持卵裂沟的位置，并且这些信号独立于其他沟组分控制肌球蛋白II的定位。
Completion of cell division during cytokinesis requires temporally and spatially regulated communication from the microtubule cytoskeleton to the actin cytoskeleton and the cell membrane. We identified a specific inhibitor of nonmuscle myosin II, blebbistatin, that inhibited contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. Using blebbistatin and other drugs, we showed that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis. Continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components.