PHAGOTROPHY AND 2 NEW STRUCTURES IN THE MALARIA PARASITE PLASMODIUM-BERGHEI
PHAGOTROPHY AND 2 NEW STRUCTURES IN THE MALARIA PARASITE PLASMODIUM-BERGHEI
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DOI：
10.1083/jcb.6.1.103
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发表时间：
1959-01-01
期刊：
影响因子：
--
通讯作者：
TRAGER, W
中科院分区：
文献类型：
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作者：
RUDZINSKA, MA;TRAGER, W
将从感染伯氏疟原虫的大鼠收集的血液离心，并将沉淀物在含有4.9%蔗糖的1%缓冲OsO 4中固定1小时，将材料包埋在甲基丙烯酸正丁酯中，并将所得块切片用于电子显微镜检查。寄生虫被发现包含，在几乎所有的部分，椭圆体的密度和结构相同的宿主细胞质。在许多电子显微照片中发现这些体与宿主细胞质之间的连续性，表明这些体是由寄生虫的双层质膜内陷形成的。通过这种方式，宿主细胞通过吞噬作用被整合到寄生虫内的食物泡中。血红素是血红蛋白消化后的残留物，在食物泡内从未观察到，但在食物泡周围的小泡中观察到，有时与食物泡相连，这些小泡从食物泡本身被夹断，是血红蛋白消化的部位。活性双界膜不仅负责食物泡的形成，而且还负责两个新结构的存在。一种是由2 ~ 6个起源于质膜的同心双波纹膜组成。由于在伯氏疟原虫中没有发现典型的线粒体，因此推测同心结构执行线粒体功能。另一种结构表现为香肠状的空泡，被2层厚度、密度和间距与身体界膜相同的膜包围。胞质内有丰富的内质网小泡和Palade’s小颗粒。细胞核密度低，被双层膜包裹。宿主细胞（网织红细胞）有线粒体，线粒体上有许多线粒体嵴。在许多感染和完整的网织红细胞中，铁蛋白被发现存在于空泡、线粒体、小管或散在细胞质中。
Blood collected from rats infected with Plasmodium berghei was centrifuged and the pellet was fixed for 1 hour in 1% buffered OsO4 with 4.9% sucrose, the material was embedded in n-butyl methacrylate and the resulting blocks sectioned for electron microscopy. The parasites were found to contain, in almost all sections, oval bodies of the same density and structure as the host cytoplasm. Continuity between these bodies and the host cytoplasm was found in a number of electron micrographs, showing that the bodies are formed by invagination of the double plasma membrane of the parasite. In this way the host cell is incorporated by phagotrophy into food vacuoles within the parasite. Hematin, the residue of hemoglobin digestion, was never observed inside the food vacuole but in small vesicles lying around it and sometimes connected with it. The vesicles are pinched off from the food vacuole proper and are the site of hemoglobin digestion. The active double limiting membrane is responsible not only for the formation of food vacuoles but also for the presence of 2 new structures. One is composed of 2 to 6 concentric double wavy membranes originating from the plasma membrane. Since no typical mitochondria were found in P. berghei, it is assumed that the concentric structure performs mitochondrial functions. The other structure appears as a sausage-shaped vacuole surrounded by 2 membranes of the same thickness, density, and spacing as the limiting membrane of the body. The cytoplasm of the parasite is rich in vesicles of endoplasmic reticulum and Palade''s small particles. Its nucleus is of low density and encased in a double membrane. The host cells (reticulocytes) have mitochondria with numerous cristae mito-chondriales. In many infected and intact reticulocytes ferritin was found in vacuoles, mitochondria, canaliculi, or scattered in the cytoplasm.