Revisiting the scientific method to improve rigor and reproducibility of immunohistochemistry in reproductive science.
Revisiting the scientific method to improve rigor and reproducibility of immunohistochemistry in reproductive science.
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重新审视提高生殖科学中免疫组织化学的严谨性和重现性的科学方法。
DOI：
10.1093/biolre/ioy094
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发表时间：
2018
影响因子：
3.6
通讯作者：
Duncan,FrancescaE
中科院分区：
文献类型：
--
作者：
Manuel,SharrónL;Johnson,BrianW;Frevert,CharlesW;Duncan,FrancescaE
免疫组织化学（IHC）是一种强大的科学工具，通过它可以可视化组织内的细胞成分，这种方法已经并将继续成为许多生殖生物学家的主要方法。如果进行和解释正确，免疫组化可以提供大量信息，但研究表明，在免疫组化实验中普遍使用和报告适当对照的情况很低。这种科学方法的遗漏可能导致数据缺乏严谨性和可重复性。在这篇社论中，我们强调了IHC控制的关键概念，并描述了我们的领域与组织化学学会合作的机会，作为研究人员、作者、特别审稿人、编委会成员和主编，广泛采用他们的IHC指南。随着新技术的出现，这种跨专业的社会互动将确保我们产生最高质量的数据，这些数据仍然依赖于经典组织学和免疫组织化学原理的基础。
Immunohistochemistry (IHC) is a robust scientific tool whereby cellular components are visualized within a tissue, and this method has been and continues to be a mainstay for many reproductive biologists. IHC is highly informative if performed and interpreted correctly, but studies have shown that the general use and reporting of appropriate controls in IHC experiments is low. This omission of the scientific method can result in data that lack rigor and reproducibility. In this editorial, we highlight key concepts in IHC controls and describe an opportunity for our field to partner with the Histochemical Society to adopt their IHC guidelines broadly as researchers, authors, ad hoc reviewers, editorial board members, and editors-in-chief. Such cross-professional society interactions will ensure that we produce the highest quality data as new technologies emerge that still rely upon the foundations of classic histological and immunohistochemical principles.