Vector Analysis of Cytoskeletal Structural Tension and the Mechanisms that Underpin Spectrin-Related Forces in Pyroptosis
Vector Analysis of Cytoskeletal Structural Tension and the Mechanisms that Underpin Spectrin-Related Forces in Pyroptosis
复制标题
细胞骨架结构张力的矢量分析以及细胞焦亡中血影蛋白相关力的支撑机制。
DOI：
10.1089/ars.2017.7366
复制
发表时间：
2018-05-30
影响因子：
6.6
通讯作者：
Liu, Xiaoguang Margaret
中科院分区：
文献类型：
--
作者：
Chen, Tingting;Guo, Yichen;Liu, Xiaoguang Margaret
旨在
Pyroptotic细胞的特征在于由基于血影蛋白的膜骨架和细胞间竞争性张力活动介导的细胞膜的胞浆肿胀、膜起泡和崩解。基于血影蛋白的膜骨架通过调节细胞间张力参与膜组织。使用遗传编码的张力传感器，以实现非侵入性的力测量结构蛋白，我们研究了细胞骨架结构张力如何影响血浆形态的变化，在pyroptosis和细胞骨架结构张力的调节机制，支持pyroptosis。
结果
结果表明，增加的血影蛋白张力是由渗透膨胀。收缩蛋白张力的升高与微丝和微管协同传递的收缩张力密切相关。然而，在pyroptotic细胞的收缩蛋白张力的增加是由MF和MT力拮抗控制。与MF张力不同，MT外张力参与膜泡的形成。由向内MF力引起的Spectrin张力抵抗热变性肿胀。MF和MT结构的稳定对细胞张力和细胞凋亡变形的影响不大。热裂解诱导的细胞骨架结构张力高度依赖于钙信号和活性氧的产生。阻断膜孔、非选择性离子通量或消除半胱天冬酶-1裂解导致与细胞凋亡失败相关的结构力缓解。创新与结论：这些数据表明，亚细胞张力，在幅度和矢量方面，是通过调解肿胀和起泡和结构张力，特别是MT力的消除，可能会导致焦亡抑制焦亡的组成部分。抗氧化剂。氧化还原信号。00，000-000。
AIMS
Pyroptotic cells are characterized by plasma swelling, membrane blebbing, and disintegration of the cell membrane mediated by spectrin-based membrane skeleton and intercellular competitive tension activities. The spectrin-based membrane skeleton is involved in membrane organization through the regulation of intercellular tension. Using genetically encoded tension sensors to attain noninvasive force measurements in structural proteins, we investigated how cytoskeletal structural tension influences changes in plasma morphology during pyroptosis and the regulatory mechanism of cytoskeletal structural tension that underpins pyroptosis.
RESULTS
The results indicate that increasing spectrin tension is caused by osmotic swelling. Hightened tension of spectrin was closely associated with the shrink tension transmitted synergistically by microfilaments (MFs) and microtubules (MTs). However, the increment of spectrin tension in pyroptotic cells was controlled antagonistically by MF and MT forces. Different from MF tension, outward MT forces participated in the formation of membrane blebs. Spectrin tension caused by inward MF forces resisted pyroptosis swelling. Stabilization of MF and MT structure had little influence on intracellular tension and pyroptosis deformation. Pyroptosis-induced cytoskeletal structural tension was highly dependent on calcium signaling and reactive oxygen species generation. Blocking of membrane pores, nonselective ion flux, or elimination of caspase-1 cleavage resulted in the remission of structural forces associated with pyroptosis failure. Innovation and Conclusions: The data suggest that subcellular tension, in terms of magnitude and vector, is integral to pyroptosis through the mediation of swelling and blebbing and the elimination of structural tension, especially MT forces, may result in pyroptosis inhibition. Antioxid. Redox Signal. 00, 000-000.