ESTABLISHMENT AND CHARACTERIZATION OF HUMAN CELL-LINES FROM A SEROUS PAPILLARY ADENOCARCINOMA OF THE ENDOMETRIUM
ESTABLISHMENT AND CHARACTERIZATION OF HUMAN CELL-LINES FROM A SEROUS PAPILLARY ADENOCARCINOMA OF THE ENDOMETRIUM
复制标题
DOI：
10.1006/gyno.1994.1191
复制
发表时间：
1994-08-01
影响因子：
4.7
通讯作者：
NODA, T
中科院分区：
文献类型：
--
作者：
HIRAI, Y;KAWAGUCHI, T;NODA, T
源自人浆液性乳头状子宫内膜腺癌的两种细胞系 SPAC-1-L 和 SPAC-1-S 已被建立，并作为单层培养物重复传代培养 3 年多，历经 144 代。两种细胞系中的每一种都是源自一种原始肿瘤的亚系。它们是从原代培养皿中独立传代培养的，没有任何克隆亚群的尝试，并进一步表征了形态、生长动力学、肿瘤标志物产生、核型和致瘤性。这些研究表明，SPAC-1-L和-S细胞系在一些细胞特性方面彼此不同，例如形态特征、生长动力学和肿瘤标志物产生。与之前文献报道的浆液性乳头状子宫内膜癌细胞系相比，我们的两种细胞系在裸鼠上更具致瘤性（100%），这可能与原始肿瘤异常侵袭性的生物学行为有关。此外，植入的裸鼠肿瘤，特别是源自SPAC-1-S细胞的肿瘤，保留了原始肿瘤的许多特征，并且在组织学上与原始浆液性乳头状腺癌非常相似。由于浆液性乳头状癌是子宫内膜腺癌的一种临床侵袭性组织学变异，预后明显较差，因此这些 SPAC-1-L 和 -S 细胞系应该在未来研究该肿瘤的极具侵袭性的生物学行为和独特形态的过程中发挥作用。 (C) 1994 年学术出版社
Two cell lines, SPAC-1-L and SPAC-1-S, derived from a human serous papillary endometrial adenocarcinoma, have been established and repetitively subcultured as a monolayer culture for over 3 years through 144 passage generations. Each of the two cell lines is a subline derived from one original tumor. They have been independently subcultured from the primary culture dishes without any attempt to clone subpopulations and were further characterized with regard to morphology, growth kinetics, tumor marker production, karyotype, and tumorigenicity. These studies indicated that the SPAC-1-L and -S cell lines are different from each other in some cell properties such as morphological character, growth kinetics, and tumor marker productions. Compared with cell lines from a serous papillary endometrial carcinoma that has been previously reported in the literature, our two cell lines are more tumorigenic on nude mice (100%), which possibly correlates with the unusually aggressive biological behavior of the original tumor. In addition, implanted nude mouse tumors, especially the tumor derived from SPAC-1-S cells, retained many characteristics of the original tumor and histologically closely resembled the original serous papillary adenocarcinoma. Since serous papillary carcinoma is a clinically aggressive histological variant of endometrial adenocarcinoma with a significantly poor prognosis, these SPAC-1-L and -S cell lines should prove useful in future studies of the extremely aggressive biological behavior and unique morphology of this tumor. (C) 1994 Academic Press, Inc.