Murine high-fat diet and laser photochemical model of basal deposits in bruch membrane
Murine high-fat diet and laser photochemical model of basal deposits in bruch membrane
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DOI：
10.1001/archopht.119.11.1643
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发表时间：
2001-11-01
影响因子：
--
通讯作者：
Grossniklaus, HE
中科院分区：
文献类型：
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作者：
Dithmar, S;Sharara, NA;Grossniklaus, HE
目的：研究高脂饮食和激光光化学损伤对小鼠Bruch膜的组织学、组织化学和超微结构的影响。第1组包括正常饮食的2月龄小鼠;第2组包括正常饮食的8月龄小鼠;第3组包括高脂肪饮食的8月龄小鼠;第4组和第5组分别包括正常饮食或高脂肪饮食的8月龄小鼠，其一只眼睛接受氩蓝激光（488 nm）的激光应用。处死小鼠并测量血浆脂质水平。用标准电子显微镜、非酯化胆固醇（UC）和酯化胆固醇（EC）的菲力平组织化学以及锇-单宁酸-苯二胺法保存细胞外脂质颗粒（Lipoprotein.Results）对眼睛进行检查。第2组的Bruch膜比第1组厚（P=.04），第3组的Bruch膜比第2组厚（P=.003）。第3组中的所有眼睛均显示电子透明碎片积聚。没有组织化学和超微结构证据表明该物质代表累积的UC或EC。第5组9只激光损伤眼中有7只在Bruch膜上积累了基底层存款（BlamD）样物质（P= 0.02）。高脂肪饮食。这种碎片与基底线性沉积物有一些相似之处，尽管碎片不像基底线性沉积物那样在视网膜色素上皮的基底膜外部形成离散层。这些沉积物似乎不是UC或EC。视网膜色素上皮的激光光化学损伤可导致具有电子透明碎片的眼睛出现BlamD样沉积物。在这个模型中的BlamD样存款是类似的基础层存款发生在年龄相关性黄斑degeneration.Clinical Relevance：这是一个动物模型的超微结构BlamD样物质在布鲁赫膜是非常相似的存款发生在年龄相关性黄斑变性。
Objective: To examine the histologic, histochemical, and ultrastructural changes in Bruch membrane in mice on a high-fat diet with and without laser photochemical injury.Methods: Five groups of C57BL/6 mice were studied. Group 1 included 2-month-old mice on a normal diet; group 2 included 8-month-old mice on a normal diet; group 3 included 8-month-old mice on a high-fat diet; groups 4 and 5 included 8-month-old mice on a normal diet or high-fat diet, respectively, that underwent laser application of one eye with argon blue laser (488 nm). The mice were killed and plasma lipid levels were measured. The eyes were examined by standard electron microscopy, filipin histochemistry for unesterified cholesterol (UC) and esterified cholesterol (EC), and the osmium-tannic acid-phenylenediamine method for preserving extracellular lipid particles.Results: The plasma cholesterol level was significantly higher in the mice on the high-fat diet than the controls (P < .001). Bruch membrane was thicker in group 2 than group 1 (P=.04) and group 3 had a thicker Bruch membrane than group 2 (P=.003). All eyes in group 3 exhibited accumulation of electron-lucent debris. There was no histochemical and ultrastructural evidence that this material represented accumulated UC or EC. Seven of 9 laser-injured eyes in group 5 accumulated basal laminar deposit (BlamD)-like material in Bruch membrane (P=.02).Conclusions: Electron-lucent debris accumulates in murine Bruch membrane, and the amount correlates with age. and high-fat diet. This debris has some similarities with basal linear deposits, although the debris does not form a discrete layer external to the basement membrane of the retinal pigment epithelium as occurs in basal linear deposits. These deposits do not appear to be UC or EC. Laser photochemical injury of the retinal pigment epithelium may result in the appearance of BlamD-like deposits in eyes with electron-lucent debris. The BlamD-like deposits in this model are similar to the basal laminar deposits that occur in age-related macular degeneration.Clinical Relevance: This is an animal model of ultrastructural BlamD-like material in Bruch membrane that is very similar to the deposits that occur in age-related macular degeneration.