Experimental metabonomic model of dietary variation and stress interactions.
Experimental metabonomic model of dietary variation and stress interactions.
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DOI：
10.1021/pr0504182
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发表时间：
2006-06
影响因子：
4.4
通讯作者：
Yulan Wang;E. Holmes;Huiru Tang;J. Lindon;N. Sprenger;M. Turini;G. Bergonzelli;L. Fay;S. Kochhar-S.-Koc
中科院分区：
文献类型：
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作者：
Yulan Wang;E. Holmes;Huiru Tang;J. Lindon;N. Sprenger;M. Turini;G. Bergonzelli;L. Fay;S. Kochhar-S.-Koc
新生大鼠在适度的母鼠分离期所产生的压力被认为会导致中枢神经系统的永久性变化，并在以后的生活中引起疾病。人们还认为，在饲料中补充长链多不饱和脂肪酸（LC-PUFAs）可能会改善应激的影响。在大鼠（出生后2-14天）中研究了早期母亲分离应激的代谢后果，无论是单独的还是在3-4月龄时与继发性急性避水应激联合。还评估了富含lc - pufa的饮食干预对应激动物的影响。利用血浆1H核磁共振波谱和多元模式识别技术评估代谢生化的系统性变化。对应激的生化反应表现为总脂蛋白水平降低，氨基酸、葡萄糖、乳酸、肌酸和柠檬酸水平升高。继发性急性避水应激也引起血浆中o -乙酰糖蛋白水平升高。饮食中LC-PUFAs的富集并没有改变对应激的代谢反应，但确实导致了脂蛋白谱的改变。这项研究表明，不同的应激源类型会导致一些共同的全身代谢反应，包括能量和肌肉代谢的变化，但这些反应不能通过饮食干预来逆转。
Stress in the form of moderate periods of maternal separation of newborn rats has been postulated to cause permanent changes in the central nervous system and diseases in later life. It is also considered that dietary supplementation with long chain polyunsaturated fatty acids (LC-PUFAs) can potentially ameliorate the effects of stress. The metabolic consequences of early life maternal separation stress were investigated in rats (2-14 days after birth), either alone or in combination with secondary acute water avoidance stress at 3-4 months of age. The effect of a LC-PUFA-enriched dietary intervention in stressed animals was also assessed. Systematic changes in metabolic biochemistry were evaluated using 1H nuclear magnetic resonance spectroscopy of blood plasma and multivariate pattern recognition techniques. The biochemical response to stress was characterized by decreased levels of total lipoproteins and increased levels of amino acids, glucose, lactate, creatine, and citrate. Secondary acute water avoidance stress also caused elevated levels of O-acetyl glycoproteins in blood plasma. LC-PUFAs dietary enrichment did not alter the metabolic response to stress, but did result in a modified lipoprotein profile. This work indicates that the different stressor types resulted in some common systemic metabolic responses that involve changes in energy and muscle metabolism, but that they are not reversible by dietary intervention.