Trait to gene analysis reveals that allelic variation in three genes determines seed vigour.
Trait to gene analysis reveals that allelic variation in three genes determines seed vigour.
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DOI：
10.1111/nph.14102
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发表时间：
2016-12
期刊：
影响因子：
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通讯作者：
Finch-Savage WE
中科院分区：
文献类型：
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作者：
Morris K;Barker GC;Walley PG;Lynn JR;Finch-Savage WE
可预测的幼苗建立对于资源有效和成本效益的作物生产至关重要；
我们在农作物胸腺橄榄石中使用了自然变异和细微的映射，以表明三个基因座的等位基因变异会影响橄榄石的快速发芽的关键活力特征。
在B. oleracea中鉴定出两个候选基因（SOG1）。影响影响的IC酸1（raba1） ABA含量并提供了证据表明，这是由于该基因座的ABA分解代谢基因ATCYP707A2的活性而产生的。
包含这三个基因的有益等位基因的线具有更大的种子活力。
Predictable seedling establishment is essential for resource‐efficient and cost‐effective crop production; it is widely accepted as a critically important trait determining yield and profitability. Seed vigour is essential to this, but its genetic basis is not understood. We used natural variation and fine mapping in the crop Brassica oleracea to show that allelic variation at three loci influence the key vigour trait of rapid germination. Functional analysis in both B. oleracea and the model Arabidopsis identified and demonstrated activity of genes at these loci. Two candidate genes were identified at the principal Speed of Germination QTL (SOG1) in B. oleracea. One gene BoLCVIG2 is a homologue of the alternative‐splicing regulator (AtPTB1). The other gene BoLCVIG1 was unknown, but different alleles had different splice forms that were coincident with altered abscisic acid (ABA) sensitivity. We identified a further QTL, Reduced ABscisic Acid 1 (RABA1) that influenced ABA content and provide evidence that this results from the activity of a homologue of the ABA catabolic gene AtCYP707A2 at this locus. Lines containing beneficial alleles of these three genes had greater seed vigour. We propose a mechanism in which both seed ABA content and sensitivity to it determines speed of germination.