Intestinal intermediate filament polypeptides in C. elegans: Common and isotype-specific contributions to intestinal ultrastructure and function
Intestinal intermediate filament polypeptides in C. elegans: Common and isotype-specific contributions to intestinal ultrastructure and function
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DOI：
10.1038/s41598-020-59791-w
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发表时间：
2020-02-21
影响因子：
4.6
通讯作者：
Leube, Rudolf E.
中科院分区：
文献类型：
--
作者：
Geisler, Florian;Coch, Richard A.;Leube, Rudolf E.
研究了C. elegans肠表明肠道功能和有机体健康的重要贡献。荧光IF报告基因定位于富含肌动蛋白的刷状缘下方，并高度富集于与C. elegans顶端连接映射肠粘弹性通过非接触布里渊显微镜显示，IF丰富的endotube位于之间的接口硬刷状缘和软细胞质，这表明机械缓冲功能，以处理频繁的管腔变形发生在食物摄入和运动。因此，IFB-2、IFC-2和IFD-2的消耗导致肠腔扩张，尽管IFC-1、IFD-1和IFP-1的消耗不会。功能丧失突变体的超微结构分析进一步表明，IFC-2突变体有一个稀薄的内管和IFB-2突变体完全缺乏内管。值得注意的是，几乎所有IFB-2和IFC-2缺陷的动物都能发育成有生育能力的成年动物。但发育迟缓，减少育雏大小，改变生存和微生物毒素，渗透和氧化应激的敏感性增加，在这两个突变体中看到，虽然程度不同。两者合计，我们建议，个别肠IF多肽有助于以不同的方式，内管形态发生和合作，以科普不断变化的环境。
The abundance and diversity of intermediate filaments (IFs) in the C. elegans intestine indicate important contributions to intestinal function and organismal wellbeing. Fluorescent IF reporters localize below the actin-rich brush border and are highly enriched in the lumen-enveloping endotube, which is attached to the C. elegans apical junction. Mapping intestinal viscoelasticity by contact-free Brillouin microscopy reveals that the IF-rich endotube is positioned at the interface between the stiff brush border and soft cytoplasm suggesting a mechanical buffering function to deal with the frequent luminal distortions occurring during food intake and movement. In accordance, depletion of IFB-2, IFC-2 and IFD-2 leads to intestinal lumen dilation although depletion of IFC-1, IFD-1 and IFP-1 do not. Ultrastructural analyses of loss of function mutants further show that IFC-2 mutants have a rarefied endotube and IFB-2 mutants lack an endotube altogether. Remarkably, almost all IFB-2- and IFC-2-deficient animals develop to fertile adults. But developmental retardation, reduced brood size, altered survival and increased sensitivity to microbial toxin, osmotic and oxidative stress are seen in both mutants albeit to different degrees. Taken together, we propose that individual intestinal IF polypeptides contribute in different ways to endotube morphogenesis and cooperate to cope with changing environments.