FIBRONECTIN AND INTEGRINS IN CELL-ADHESION AND MIGRATION
FIBRONECTIN AND INTEGRINS IN CELL-ADHESION AND MIGRATION
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DOI：
10.1042/bst0190830
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发表时间：
1991-11-01
影响因子：
3.9
通讯作者：
YAMADA, KM
中科院分区：
文献类型：
--
作者：
AOTA, S;NAGAI, T;YAMADA, KM
纤连蛋白、其他细胞粘附分子及其整联蛋白受体似乎在胚胎发育期间的细胞迁移、肿瘤细胞侵袭和转移以及伤口愈合中发挥重要作用（综述见[11]）。最近的研究已经确定了纤维连接蛋白中与细胞表面受体相互作用所需的关键肽识别区域。例如，Arg-Gly-Asp（RGD）序列对于纤连蛋白的中央细胞结合结构域的功能是重要的[Z-41]，而纤连蛋白可变剪接区中的Leu-Asp-瓦尔（LDV）和Arg-Glu-Asp-瓦尔序列参与胚胎神经嵴衍生物和某些淋巴细胞的细胞类型特异性粘附[5，61.相反，多种整联蛋白受体可介导细胞在纤连蛋白和其他细胞外分子上的粘附和迁移[7-131]。我们应用定点突变和单克隆抗体分析来研究细胞表面与纤连蛋白相互作用的分子机制。我们已经集中在纤连蛋白的中央细胞结合域，它介导的细胞附着，细胞扩散，和各种各样的细胞类型的迁移的细节。
Fibronectin, other cell-adhesion molecules, and their integrin receptors appear to play important roles in cell migration during embryonic development, tumour-cell invasion and metastasis, and wound healing (reviewd in [11). Recent studies have defined critical peptide-recognition regions in fibronectin that are required for its interactions with cell-surface receptors. For example, the Arg-Gly-Asp (RGD) sequence is important for the functioning of the central cell-binding domain of fibronectin [Z-41, while the Leu-Asp-Val (LDV) and Arg-Glu-Asp-Val sequences in alternatively spliced regions of fibronectin are involved in cell-type specific adhesion by derivatives of the embryonic neural crest and by certain lymphocytes [5, 61. Conversely, a variety of integrin receptors can mediate cell adhesion and migration on fibronectin and other extracellular molecules [7-131. We have applied site-directed mutagenesis and monoclonal-antibody analysis to examine the molecular mechanisms of cell-surface interactions with fibronectin. We have focused in detail on the central cell-binding domain of fibronectin, which mediates cell attachment, cell spreading, and migration of a wide variety of cell types.