A novel application for Neurospora crassa:: Progress from batch culture to a membrane bioreactor for the bioremediation of phenols
A novel application for Neurospora crassa:: Progress from batch culture to a membrane bioreactor for the bioremediation of phenols
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DOI：
10.1016/s0141-0229(01)00390-8
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发表时间：
2001-10-04
影响因子：
3.4
通讯作者：
Burton, SG
中科院分区：
文献类型：
--
作者：
Luke, AK;Burton, SG
通过静态、摇摇非固定化分批培养和毛细膜固定化生物膜，研究了粗神经孢子菌真菌系统产生氧化酶的能力及其在酚类化合物生物降解中的应用。细胞外漆酶活性在静态瓶培养中分别为10-12 U/mL （800 U/g湿质量）和1.5 U/mL （130 U/g湿质量），持续8-15天。多酚氧化酶是一种细胞内酶，在374 U/g湿质量水平下产生。在毛细管膜生物反应器中，漆酶的生产维持在10 U/mL渗透物（1080 U/g湿生物质）的水平，通常持续30-40天。两种酚类底物，苯酚和对甲酚，都是工业废水的常见成分，被选择作为模型污染物，使用n.c assa酶系统进行生物修复研究。在烧瓶培养中，在6天的时间里，从5 mM /g湿生物质溶液中分别去除18 mg对甲酚和23 mg苯酚。在同一时间内，固定化培养物被发现转化10毫克对甲酚或8毫克苯酚/克生物量。在连续反应器中固定化的生物质被发现有能力在4个月的时间内持续保持这种去除效率，而间歇式液体培养系统保持大约8-15天的活性，之后培养物不再存活。这是首次展示了固定化芒草生物膜的使用及其在酚类生物修复中的连续应用。(C) 2001爱思唯尔科学公司版权所有。
The capacity of a Neurospora crassa fungal system to produce oxidative enzymes, and their application in the biodegradation of phenolic compounds, was demonstrated in static and shaken non-immobilized batch cultures, and by capillary membrane-immobilized biofilms. Extracellular laccase activity was produced at 10-12 U/mL (800 U/g wet mass) in static flask cultures, and 1.5 U/mL (130 U/g wet mass) in shaken batch cultures, respectively, over 8-15 days. Polyphenol oxidase was shown to be produced as an intracellular enzyme, at levels of 374 U/g wet mass. The production of laccase in a capillary membrane bioreactor was sustained at a level of 10 U/mL of permeate (1080 U/g wet biomass), typically over 30-40 days.Two phenolic substrates, phenol and p-cresol, both common components of industrial effluent streams, were chosen as model pollutants for bioremediation studies using the N.crassa enzyme system. In flask cultures, 18 mg p-cresol and 23 mg phenol respectively were removed from 5 mM solutions/g wet biomass, over a 6 day period. Over the same time period, immobilized cultures were found to convert 10 mg p-cresol or 8 mg phenol/g biomass. The immobilized biomass in a continuous reactor was found to have the capacity to sustain this removal efficiency continuously for a 4-month period, whereas the batch liquid culture systems remained active for approximately 8-15 days, after which cultures were no longer viable. This is the first demonstration of the use of immobilized N.crassa biofilms and their continuous application for bioremediation of phenols. (C) 2001 Elsevier Science Inc. All rights reserved.