Identification and characterization of canine growth differentiation factor-9 and its splicing variant.
Identification and characterization of canine growth differentiation factor-9 and its splicing variant.
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DOI：
10.1016/j.gene.2012.03.003
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发表时间：
2012-05-15
期刊：
影响因子：
3.5
通讯作者：
Shimasaki S
中科院分区：
文献类型：
--
作者：
Hashimoto O;Takagi R;Yanuma F;Doi S;Shindo J;Endo H;Hasegawa Y;Shimasaki S
生长分化因子-9 (GDF-9) 是转化生长因子-β (TGF-β) 超家族的成员，仅在卵巢内的卵母细胞中表达，在哺乳动物的卵巢功能中发挥重要作用。然而，GDF-9 可能参与犬类卵巢生理学（在哺乳动物中具有独特的排卵过程）尚未得到研究。有趣的是，我们从犬卵巢总 RNA 中分离出两种通过选择性剪接产生的 cDNA 克隆。主要的长型 cDNA 与其他物种中的 GDF-9 具有共同的前体结构，而次要的短型 cDNA 在前区有 172 个氨基酸的截短。使用瞬时表达系统，我们发现长形式 cDNA 在成熟蛋白质产生方面存在缺陷，而短形式 cDNA 很容易产生成熟蛋白质。然而，短型 GDF-9 成熟结构域中一两个 N-糖基化位点的突变导致成熟蛋白生产的损失。这些结果表明成熟结构域的前结构域和 N 连接糖基化调节犬 GDF-9 的正确加工和分泌。基于 GDF-9 的生物学功能，犬 GDF-9 的这些特征可能与犬科动物独特的排卵过程存在因果关系。
Growth differentiation factor-9 (GDF-9), a member of the transforming growth factor-β (TGF-β) superfamily, is expressed exclusively in the oocyte within the ovary and plays essential roles in the ovarian function in mammals. However, a possible involvement of GDF-9 in canine ovarian physiology that has a unique ovulation process among mammals has not been studied. Interestingly, we have isolated two types of cDNA clones generated by an alternative splicing from a canine ovarian total RNA. The predominant long form cDNA shares a common precursor structure with GDF-9s in other species whereas the minor short form cDNA has a 172 amino acid truncation in the proregion. Using a transient expression system, we found that the long form cDNA has a defect in mature protein production whereas the short form cDNA readily produces mature protein. However, mutations at one or two N-glycosylation sites in the mature domain of the short form GDF-9 caused a loss in mature protein production. These results suggest that the prodomain and N-linked glycosylation of the mature domain regulate proper processing and secretion of canine GDF-9. Based on the biological functions of GDF-9, these characteristics of canine GDF-9 could be causatively linked to the unique ovulation process in the Canidae.
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影响因子：
4.8
作者：
Dragovic, RA;Ritter, LJ;Gilchrist, RB
通讯作者：
Gilchrist, RB
DOI：
10.1073/pnas.93.12.5877
发表时间：
1996-06-11
影响因子：
11.1
作者：
Bottinger, EP;Factor, VM;Sporn, MB
通讯作者：
Sporn, MB
DOI：
10.1073/pnas.180295197
发表时间：
2000-08-29
影响因子：
11.1
作者：
Elvin, JA;Yan, CN;Matzuk, MM
通讯作者：
Matzuk, MM
影响因子：
3.6
作者：
McIntosh, C. Joy;Lun, Stan;Juengel, Jennifer L.
通讯作者：
Juengel, Jennifer L.
影响因子：
6.7
作者：
Kovanci, Ertug;Rohozinski, Jan;Carson, Sandra A.
通讯作者：
Carson, Sandra A.