CFU-GM-derived cells form osteoclasts at a very high efficiency
CFU-GM-derived cells form osteoclasts at a very high efficiency
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DOI：
10.1006/bbrc.1999.2042
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发表时间：
2000-01-27
影响因子：
3.1
通讯作者：
Roodman, GD
中科院分区：
文献类型：
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作者：
Menaa, C;Kurihara, N;Roodman, GD
粒细胞-巨噬细胞祖细胞（CFU-GM）是一种多能细胞，可以分化为破骨细胞（OCLs）、巨噬细胞或粒细胞，然而，CFU-GM有效形成OCLs的相对潜力尚不清楚。在本报告中，我们证明了粒细胞-巨噬细胞集落形成单位（CFU-GM）衍生的细胞是一种容易获得的高度纯化的人OCL前体来源，当与RANK配体（RANKL）、巨噬细胞集落刺激因子（M-CSF）和地塞米松一起培养时，形成的OCL效率非常高（大于90%），形成的OCL具有高骨吸收活性，并且在牙本质片上每个OCL形成多个吸收腔隙。同样，小鼠骨髓源性CFU-GM在经RANKL、M-CSF和地塞米松处理后也能高效率（约80%）形成ocl。相比之下，在这些条件下，更多的巨噬细胞集落形成单位（CFU-M）来源的细胞形成很少的ocl， (C) 2000学术出版社。
The granulocyte-macrophage progenitor (CFU-GM) is a multipotent cell that can differentiate to osteoclasts (OCLs), macrophages, or granulocytes, However, the relative potential of CFU-GM to efficiently form OCLs is unknown. In this report we demonstrate that granulocyte-macrophage colony-forming unit (CFU-GM)-derived cells represent an easily obtainable highly purified source of human OCL precursors that form OCLs at very high efficiency (greater than 90%) when cultured with RANK ligand (RANKL), macrophage colony-stimulating factor (M-CSF), and dexamethasone, The OCLs that formed have high bone-resorbing activity and form multiple resorption lacunae per OCL on dentin slices. Similarly, murine marrow-derived CFU-GM also formed OCLs at a high efficiency (>80%) when treated with RANKL, M-CSF, and dexamethasone. In contrast, more committed macrophage colony-forming unit (CFU-M)-derived cells form few OCLs under these conditions, (C) 2000 Academic Press.