Open-source do-it-yourself multi-color fluorescence smartphone microscopy
Open-source do-it-yourself multi-color fluorescence smartphone microscopy
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DOI：
10.1364/boe.8.005075
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发表时间：
2017-11-01
影响因子：
3.4
通讯作者：
Shih, Wei-Chuan
中科院分区：
文献类型：
--
作者：
Sung, Yulung;Campa, Fernando;Shih, Wei-Chuan
荧光显微镜是细胞和微生物研究的重要技术。将这种技术转换到智能手机上可以实现特别强大的应用程序，例如现场分析，按需监测和即时诊断。目前的荧光智能手机显微镜设置需要精确的照明和成像对准，这完全限制了其广泛采用。我们报告了一种多色荧光智能手机显微镜，具有类似隐形眼镜的附加透镜和滑片发射全内反射引导照明，可用于研究荧光显微镜中的三项常见任务：自发荧光、荧光染色和免疫荧光。这种开源、简单且具有成本效益的设计具有自己动手做荧光智能手机显微镜的潜力。(C)2017美国光学学会。
Fluorescence microscopy is an important technique for cellular and microbiological investigations. Translating this technique onto a smartphone can enable particularly powerful applications such as on-site analysis, on-demand monitoring, and point-of-care diagnostics. Current fluorescence smartphone microscope setups require precise illumination and imaging alignment which altogether limit its broad adoption. We report a multi-color fluorescence smartphone microscope with a single contact lens-like add-on lens and slide-launched total-internal-reflection guided illumination for three common tasks in investigative fluorescence microscopy: autofluorescence, fluorescent stains, and immunofluorescence. The open-source, simple and cost-effective design has the potential for do-it-yourself fluorescence smartphone microscopy. (C) 2017 Optical Society of America.